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pCMV HA hRB delta CDK + T821

Plasmid

#58920

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T821 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T821 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T826

Plasmid

#58921

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T826 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T826 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S249

Plasmid

#58908

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S249 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S249 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pDN-G1Tt

Plasmid

#44509

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
tetR

Use

Synthetic Biology; Expression regulator/reporter

Tags

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pCGN-BCL-6Δ (pCGN-BCL6-delta)

Plasmid

#40338

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

BCL-6Δ (BCL6 Human)

Use

Tags

Expression

Mammalian

Mutation

Missing four of six zinc fingers (see note below)…

Promoter

CMV

Available since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only
pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

Plasmid

#63886

Purpose

An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized GCaMP3(wt)

Use

AAV

Tags

Expression

Mutation

Promoter

human SYN1

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRb delta CDK + S230

Plasmid

#58907

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S230 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T252

Plasmid

#58910

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T252 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL

Plasmid

#63884

Purpose

An AAV packaging vector that expresses ER-retained GCaMP3 under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized GCaMP3(wt)

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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P2061

Plasmid

#186299

Purpose

Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter

Depositor

Joerg Stelling

Article

Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10

Inserts

LexA-HBD-B112
Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

2xLexop-minimalCyc1 and PACT1

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTYB-pLAC-I68S/I73S

Plasmid

#48728

Purpose

Bacterial expression of Lacritin Proteins with Mutation at protein 68 and 73 from Isoleucine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Use

Tags

Intein

Expression

Bacterial

Mutation

Isoleucine 68 and 73 to Serine

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB-pLAC-L108S

Plasmid

#48725

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 108 from Leucine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Use

Tags

Intein

Expression

Bacterial

Mutation

Leucine 108 to Serine

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-N71

Plasmid

#48720

Purpose

Bacterial expression of Lacritin protein with 70 AA removed from the N-terminus

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Use

Tags

Intein

Expression

Bacterial

Mutation

Deleted first 71 amino acids of mature lacritin w…

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-N45

Plasmid

#48718

Purpose

Bacterial expression of Lacritin protein with 45 AA removed from N terminus

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Use

Tags

Intein

Expression

Bacterial

Mutation

Deleted first 45 amino acids of mature lacritin w…

Promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

Plasmid

#208046

Purpose

Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

RNF4 (RNF4 Human)

Use

Lentiviral

Tags

BirAopt

Expression

Mutation

Promoter

EFS

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-GJA1-20k-N1

Plasmid

#49861

Purpose

Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

GJA1-20k (GJA1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

GJA1-20k corresponds to residues 213 to 382 to Co…

Promoter

CMV

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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opto-E-cad GFP

Plasmid

#203327

Purpose

Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

Depositor

Seraphine Wegner

Article

Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

Insert

Photoswitchable - E-cadherin with GFP tag (CDH1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Addition of Aslov2 domain at T133 of human E-cadh…

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

Plasmid

#208041

Purpose

Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

TurboID

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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N-Flag-IPO9_Lenti SFFV Puro WPRE

Plasmid

#217916

Purpose

Express IPO9 in mammalian cells

Depositor

Benjamin Cravatt

Article

Ogasawara et al Cell Chem Biol. 2024 Nov 7:S2451-9456(24

Insert

importin 9 (IPO9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEX-SP-YFP-STIM1(D76A)

Plasmid

#18859

Depositor

Tobias Meyer

Article

Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

Insert

Stromal interaction molecule 1 (STIM1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

D76A mutation - changed aspartate 76 to alanine. …

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only