We narrowed to 2,282 results for: mt

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CFP-GIV-CT WT

Plasmid

#69532

Purpose

Mammalian expression of The CFP-tagged GIV-CT biosensors

Depositor

Pradipta Ghosh

Article

Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

Insert

GIV (CCDC88A Human)

Tags

CFP

Expression

Mammalian

Mutation

C terminal amino acids S1660-S1870 of human GIV

Promoter

CMV

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1820

Plasmid

#154321

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC (LoxP)^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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ApoE2_mCh-SspB (pBS1142)

Plasmid

#185324

Purpose

For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE2

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1759

Plasmid

#154315

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::TEV::AID*::3xFLAG for NT slot

Use

CRISPR

Expression

Worm

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV ShadowG-AURKA-mTurq2

Plasmid

#157768

Purpose

Expression of AuroraA kinase biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and shadowG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

Plasmid

#185328

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA_81-236-FLAG

Tags

FLAG

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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GST-SF3B2 wild type aa 401-550 fragment

Plasmid

#67615

Purpose

bacterial expression of wild type GST-SF3B2 fragment (401-550)

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

splicing factor 3b subunit 2 (SF3B2 Human)

Tags

GST

Expression

Bacterial

Mutation

fragment of amino acids 401-550

Promoter

tac

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pJW1886

Plasmid

#154333

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I^SEC (Lox511I)^TEV::degron::3xFLAG

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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AIDx-ID-1058

Plasmid

#135347

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-1058-UGI

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEMAX-NLS-P2A_BLAST

Plasmid

#135355

Purpose

Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-2XNLS

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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