We narrowed to 8,691 results for: BLI;

1. pET-MBP-Geo_st

   Plasmid

   #87700

   Purpose

   Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

   Depositor

   Jennifer Doudna

   Insert

   GeoCas9
   

   Use

   Tags

   10xHis-MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_Efla_FLEX_APEX2-eGFPf

   Plasmid

   #171939

   Purpose

   Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein

   Depositor

   David Berson

   Insert

   APEX2-eGFPf
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG-mito-dsRED2

   Plasmid

   #173071

   Purpose

   mitochondria targeted dsRED2 expressed off of CAG promoter

   Depositor

   Thomas Schwarz

   Insert

   Mito
   

   Use

   Lentiviral

   Tags

   dsRED

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE.is2-mOpn

   Plasmid

   #74411

   Purpose

   Expresses mouse Osteopontin from transcript tv2 in mammalian cells

   Depositor

   Georgios Stathopoulos

   Insert

   mouse Opn (Spp1 Mouse)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   MMLV

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB Neo* PB

   Plasmid

   #138413

   Purpose

   Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

   Depositor

   Mario Capecchi

   Insert

   Neo*
   

   Use

   Mouse Targeting; Piggybac

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GuideVector_BbsI

   Plasmid

   #110692

   Purpose

   For rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.

   Depositor

   Douglas Mahoney

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPLICSFAST-ER-MT-P2A

   Plasmid

   #214892

   Purpose

   Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-MT-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX4T3-Stx3(1-265)

   Plasmid

   #100573

   Purpose

   Expresses cytoplasmic domain of Stx3(1-265) with a GST tag on the N-term.

   Depositor

   Thomas Weimbs

   Insert

   Stx3-1-265 (Stx3 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   No transmembrane domain, residues 4-264 present.

   Promoter

   tac

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR_Hu_BIRC5(WT)

   Plasmid

   #136351

   Purpose

   Human BIRC5 cDNA in Gateway pENTR

   Depositor

   Guillermo de Cárcer

   Insert

   BIRC5 (BIRC5 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBacMam2_N_His8_eGFP_TEV

    Plasmid

    #178806

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    His8, eGFP, TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEV-G2-Km ymScarletI

    Plasmid

    #193960

    Purpose

    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeast

    Depositor

    Bas Teusink

    Insert

    ymScarletI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTCF200 Mouse beta-2 microglobulin

    Plasmid

    #180462

    Purpose

    E. coli expression of class I MHC light chain

    Depositor

    John Altman

    Insert

    Mouse beta-2 microglobulin
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Tubulin-6

    Plasmid

    #62736

    Purpose

    Localization: Microtubules, Excitation:488, Emission:507

    Depositor

    Michael Davidson

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pLIX403-EYFP-ccdB-Puro

    Plasmid

    #158549

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLIX403-EYFP-ccdB-Blast

    Plasmid

    #158551

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid

    #106477

    Purpose

    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiGuide-10X-CS1-3'-puro

    Plasmid

    #212011

    Purpose

    Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)

    Depositor

    Danwei Huangfu

    Insert

    gRNA scaffold
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBacMam5_N_Flag_His8_eGFP_3C

    Plasmid

    #178816

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    Flag, His8, eGFP, 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. ecALDH

    Plasmid

    #67921

    Purpose

    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Insert

    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBacMam6_C_3C_His8_Stop

    Plasmid

    #178810

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    3C, His8, Stop codon

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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