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pJH4.67

Plasmid

#26948

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

gamma-tubulin (tbg-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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p246 eSpCas9_2gRNAs_mH11

Plasmid

#164854

Purpose

gRNA vector for targeting mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

gRNAs for targeting mouse H11 locus

Expression

Mammalian

Promoter

U6

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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372 pTol2-fli1ep:EGFPDest

Plasmid

#73491

Purpose

Tol2 vector with fli1ep element upstream of EGFP, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:EGFP-attR1-attR2

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA_DiCre_59.F2

Plasmid

#60207

Purpose

Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Human, Synthetic, Bacteriophage P1)

Tags

FKBP12 and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 19-59 of the humanized version of Cre…

Promoter

CMV

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFB2C

Plasmid

#195285

Purpose

Bicistronic HSV-TK and Hygromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A Hygro

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pKM19

Plasmid

#123655

Purpose

Encodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cells

Depositor

Kevin Maringer

Article

Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

Insert

Firefly luciferase

Use

Luciferase

Expression

Insect

Promoter

pIEx

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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2PH-PLCd-APEX2-P2A-mKate2

Plasmid

#71284

Purpose

Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2PH-PLCd-APEX2-P2A-mKate2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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mito-EcSTH

Plasmid

#211921

Purpose

Mitochondrial localization of EcSTH

Depositor

Binghui Li

Article

Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

Insert

mito-EcSTH

Use

Lentiviral

Tags

3xFLAG

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-CKS1

Plasmid

#177013

Purpose

Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CKS1 (CKS1B Human, Synthetic)

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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BC1177-pHR-SFFV-GFP(1-10)-NLS

Plasmid

#164039

Purpose

Expression of GFP1-10 in mammalian cells

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

GFP(1-10)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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eMagAF-EGFP-Mito

Plasmid

#162243

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagAF-EGFP-OMP25

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST_FUS_G2A_R521H

Plasmid

#26381

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Tags

FLAG-HA

Expression

Mammalian

Mutation

R521H

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pHPK1212

Plasmid

#8834

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

tobacco vein mottling virus protease, catalytic domain

Tags

MBP

Expression

Bacterial

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pCAT204

Plasmid

#134881

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-mYFP-hphMX6

Plasmid

#105155

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-hphMX6

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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NpuDnaEc-cGCaMP2

Plasmid

#34851

Depositor

Kevin Truong

Article

Wong et al Cell Calcium. 2011 Nov 29.

Insert

NpuDnaEc-cGCaMP2

Expression

Bacterial and Mammalian

Promoter

CMV, T7 lac

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pET-UbcH10-DN

Plasmid

#8502

Depositor

Joan Ruderman

Article

Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.

Insert

UbcH10 (UBE2C Human)

Tags

His

Expression

Bacterial

Mutation

Dominant Negative, C114S

Available Since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pGEMHE-mCherry-mTrim21ΔPRYSPRY

Plasmid

#105524

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Mutation

deletion of PRYSPRY domain (aa1-281)

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_NES_C1

Plasmid

#85062

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-NM

Plasmid

#1127

Depositor

Susan Lindquist

Article

Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

Insert

NM (SUP35 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only