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D4XIR1

Plasmid

#163200

Purpose

Inducible expression of UniProt identifier D4XIR1

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

D4XIR1

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pZA70

Plasmid

#174784

Purpose

RNAi

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

Intron-containing hairpin RNA (ihpRNA)-NbJIM2

Use

RNAi

Mutation

WT

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huMlCas12a

Plasmid

#174678

Purpose

Expresses 6xHis-MBP tagged MlCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huMlCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huBfCas12a

Plasmid

#174672

Purpose

Expresses 6xHis-MBP tagged BfCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huBfCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pHCKAn-yibDp-Q-GRFT

Plasmid

#158748

Purpose

low phosphate yibDp induction of Q-Griffithsin

Depositor

Michael Lynch

Article

Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

Insert

yibDp Q-Griffithsin

Expression

Bacterial

Mutation

M78Q

Promoter

yibD

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHCKAn-phoBp-GRFT

Plasmid

#158746

Purpose

low phosphate phoBp induction of Griffithsin

Depositor

Michael Lynch

Article

Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

Insert

phoBp Griffithsin

Expression

Bacterial

Promoter

phoB

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-eCas9-D1135E

Plasmid

#154926

Purpose

aTc inducible eCas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

eCas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

K848A, K1003A, R1060A, D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-OptiCas9-D1135E

Plasmid

#154928

Purpose

aTc inducible OptiCas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

OptiCas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

R661A, K1003H, D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9-D1135E

Plasmid

#154931

Purpose

aTc inducible Cas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

Cas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-pheT

Plasmid

#154920

Purpose

aTc inducible gRNA targeting pheT along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting pheT

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-mqo

Plasmid

#154921

Purpose

aTc inducible gRNA targeting mqo along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting mqo

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-hisF

Plasmid

#154922

Purpose

aTc inducible gRNA targeting hisF along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting hisF

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-ampG

Plasmid

#154923

Purpose

aTc inducible gRNA targeting ampG along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting ampG

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJBL7006

Plasmid

#136945

Purpose

Malachite green aptamer, bacterial promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

malachite green aptamer (MGA)

Promoter

J23119

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pNR292

Plasmid

#79548

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP. Implements RSM with 3-input passcode behavior.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

mTagBFP

Expression

Bacterial

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pNR166

Plasmid

#79547

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMPO 51

Plasmid

#68358

Purpose

Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2). ApR, expression vector with rrnBT1T2-Pm-MCSII, ColE1 replication origin

Depositor

Eduardo Santero

Article

Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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c7-87/199_HLL

Plasmid

#63122

Purpose

Mm-cpn cysteine double mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_87/199_HLL (NEWENTRY )

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-K87C-S1…

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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c7-132/202_HLL

Plasmid

#63123

Purpose

Mm-cpn double cysteine mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_132/202_HLL (NEWENTRY )

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-Q132C-S…

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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pTC42-dmd-4

Plasmid

#44692

Depositor

Phillip Newmark

Article

Chong et al Nat Commun. 2013; 4:1814. doi: 10.1038/n

Insert

Smed-dmd-4, 3’ RACE product

Use

T/a cloning vector for dsrna generation and the g…

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only