We narrowed to 18,233 results for: nts

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pGRNA3_LacZ_B1_IIB5

Plasmid

#247081

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets both Ecoli LacZ B1 and B5 site

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_AP_Neg_pBADProm

Plasmid

#242141

Purpose

pBAD-SD8-GIII Neg; AP Neg Circuit for AraC phage-assisted evolution

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

Gene III Negative

Expression

Bacterial

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_AP_Pos_pBADProm

Plasmid

#242139

Purpose

pBAD-sd8-GIII Cassette; AP Pos Circuit for AraC phage-assisted evolution

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

Gene III

Expression

Bacterial

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_AP_GIII_Titrate

Plasmid

#242140

Purpose

PSP-LacO Cassette; AP Prop Circuit for AraC phage-assisted evolution

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

Gene III

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_SP_SD4_AraC

Plasmid

#242143

Purpose

Contains an AraC gene to assemble a selection phage missing gene III with SapI Golden Gate Assembly

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_sd2_R2_C1

Plasmid

#242149

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE variant cassette

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R2

Expression

Bacterial

Mutation

E3K, R146L, A152T, R251L

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_sd2_R5_C1

Plasmid

#242150

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE variant cassette

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R5

Expression

Bacterial

Mutation

A2E, L156I, R251H

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pYO539

Plasmid

#235752

Purpose

Protein expression of ScVPS30 and ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Expression

Yeast

Promoter

GAL-TDH3

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pART15-asB539

Plasmid

#127394

Purpose

Expresses amcyan fluorescent gene and sRNA nt 539-559

Depositor

Ryan Senger

Article

Tanniche et al Biotechnol Prog. 2023 May-Jun;39(3):e332

Insert

AmCyan

Expression

Bacterial

Promoter

PBAD

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pYO734

Plasmid

#235753

Purpose

Protein expression of ScVPS30 and ZZ-ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Tags

ZZ-3xTEV

Expression

Yeast

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO831

Plasmid

#235754

Purpose

Protein expression of ZZ-ScVPS38 (1-212F)

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 1-212F

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO69

Plasmid

#235747

Purpose

Protein expression of FL ScVPS34 in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Expression

Yeast

Mutation

S2A

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO361

Plasmid

#235748

Purpose

Protein expression of ScVPS34 HELCAT

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 HELCAT

Expression

Yeast

Mutation

aa 268-875

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO227

Plasmid

#235749

Purpose

Protein expression of ZZ-FL ScVPS30

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS30

Tags

ZZ-3xTEV

Expression

Yeast

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO832

Plasmid

#235755

Purpose

Protein expression of ScVPS30 (1-215E), untagged

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

ScVPS30

Expression

Yeast

Mutation

aa 1-215E

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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2M-T_Mce3R

Plasmid

#225726

Purpose

To express Mce3R in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGYL17_pPromALB_enhALB_FireflyLuc

Plasmid

#220283

Purpose

Firefly luciferase with minimal Albumin promoter with a 380 nt, -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

Alb Enhancer

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLZRS-panTRK-CR1-EGFP

Plasmid

#187194

Purpose

To express a hybrid Trk receptor tagged with EGFP. This Hybrid TRK receptor was designed to bind NGF, BDNF and NT-3

Depositor

Pantelis Tsoulfas

Article

O'Connell et al J Biol Chem. 2000 Mar 17;275(11):7870-7.

Insert

CR1 pan-TRK receptor

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

mo-MLV or MMLV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pNTI763 p(Z)-mCherry + UBC9 sgRNA

Plasmid

#164918

Purpose

CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(Z)-mcherry

Use

CRISPR

Expression

Yeast

Promoter

P(Z), synthetic promoter

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI743 dual-barcoded gRNA parent vector

Plasmid

#164915

Purpose

CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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