We narrowed to 3,169 results for: EED

1. phRL-TKlk-lin-41_LCS-2G

   Plasmid

   #26544

   Depositor

   Chang-Zheng Chen

   Article

   Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

   Insert

   lin-41_LCS-2G
   

   Expression

   Mammalian

   Mutation

   Seed regions of both target sites are mutated.

   Available Since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 MYC-Sumo3 WT

   Plasmid

   #48964

   Purpose

   Expresses MYC-Sumo3 Wild Type in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO3 (SUMO3 Human)
   

   Tags

   MYC (EQKLISEED)

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pPEG

   Plasmid

   #162466

   Purpose

   Entry vector for gRNA insertion in plant prime editing expression vector

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Type

   Empty backbone

   Use

   Entry vector for grna

   Promoter

   CmYLCV

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK2660

   Plasmid

   #70709

   Purpose

   Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHEE401_GV_MOD

   Plasmid

   #194874

   Purpose

   mCherry selection in Arabidopsis seeds in T1 and T2

   Depositor

   Wolfgang Busch

   Article

   Ruiz Rosquete et al Plant J. 2025 Jan;121(1):e17177. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Promoter

   egg cell-specific

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 MYC-Sumo3Q89P

   Plasmid

   #48963

   Purpose

   Expresses MYC-Sumo3Q90P mutant in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO3 (SUMO3 Human)
   

   Tags

   MYC (EQKLISEED)

   Expression

   Mammalian

   Mutation

   Mutated Q89P

   Promoter

   T7

   Available Since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pLG327

   Plasmid

   #128797

   Purpose

   Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

   Depositor

   Christopher Voigt

   Article

   Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

   Insert

   gfpmut2 (NEWENTRY Synthetic)
   

   Expression

   Bacterial

   Promoter

   Pxyl(s)

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGE1109

   Plasmid

   #202773

   Purpose

   CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

   Inserts

   FAST marker
   pRPS5a:TREX2-zCas9_ttriple
   ccdB cassette (BsaI-excisable)
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT-GFP-mCherry-3pGW

   Plasmid

   #53965

   Purpose

   Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

   Depositor

   Saeed Tavazoie

   Article

   Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

   Inserts

   GFP
   mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG223 PRKD1 3'UTR mut

    Plasmid

    #12042

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    PRKD1 3'UTR mut (PRKD1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pDGE1108

    Plasmid

    #202772

    Purpose

    CRISPR-KO in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:zCas9_ttriple
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKL2013

    Plasmid

    #172182

    Purpose

    A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.

    Depositor

    Kan Wang

    Article

    McCaw et al Front Genome Ed. 2021 Jan 11;2:622227. d

    Insert

    mCherry
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLP-pR-himar-apraR

    Plasmid

    #199996

    Purpose

    Landing Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donor

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Landing Pads
    

    Expression

    Bacterial

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

    Plasmid

    #133339

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYI001

    Plasmid

    #170833

    Purpose

    (GUSPlus::tOCS in pFP100)*

    Depositor

    Christian Fankhauser

    Article

    Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

    Insert

    GUSPlus::tOCS
    

    Expression

    Plant

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCLV3Cas-LT0

    Plasmid

    #210548

    Purpose

    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT0::mCherrySSM
    

    Expression

    Plant

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBL_DK187

    Plasmid

    #156423

    Purpose

    Insect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein
    

    Tags

    sfGFP-His

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. plIVMD156

    Plasmid

    #160934

    Purpose

    In vivo mRNA display Gateway Destination construct

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
    

    Tags

    HIS tag, 3'UTR MS2 Stem Loop

    Expression

    Yeast

    Promoter

    MET25

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKF-P14MM2AG5h

    Plasmid

    #128254

    Purpose

    Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    rtTA-Advanced::P2A::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

    Plasmid

    #133342

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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