We narrowed to 36,942 results for: kan
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Plasmid#127193PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 S12xQ (FUS Human)
TagshexahistidineExpressionBacterialMutationS12xQ: S30Q, S44Q, S48Q, S53Q, S70Q, S84Q, S89Q, …Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCALNL-eGFP-NT1-6-gix-6-NT1
Plasmid#81204Purposecontains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmidDepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
ExpressionMammalianPromoterpCBaAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
EMMA toolkit
Plasmid kit#1000000119PurposeCollection of Golden Gate vectors and standardized genetic parts for the construction mammalian expression plasmids.DepositorApplicationCloning and Synthetic BiologyVector typeMammalian ExpressionCloning typeGolden GateAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGLU_7
Plasmid#225815PurposeTn libraries Himar variant KAN backboneDepositorPublicationTypeEmpty backboneUseSynthetic BiologyMutationN/AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_128
Plasmid#225821PurposeTn libraries Tn5 variant KAN backboneDepositorPublicationTypeEmpty backboneUseSynthetic BiologyMutationN/AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3)P Probe
Plasmid#211508PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteriaDepositorAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6xHis-SNAP-PI(3,5)P2 Probe
Plasmid#211512PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteriaDepositorInsertSnxA-2xPX
Tags6xhis-SNAPExpressionBacterialAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
plRL2
Plasmid#163631PurposeOptimized M. tuberculosis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterTet-OnAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
G13-CASE
Plasmid#168127PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F125/D126 within GNA13Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CreEGFP
Plasmid#165140PurposeExpression of Cre recombinase fused with EGFP in C-terminalDepositorInsertCre recombinase
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbAW4k-loxP-TT-loxP-mRFP1
Plasmid#134405PurposeFluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.DepositorInsertW4-loxP-TT-loxP-mRFP1
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterW4 (modified T7A1)Available sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3
Plasmid#127354Purposegateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway CloningTagsGS linker, V5, and mVenusMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pScarlet-N1
Plasmid#128060PurposeMammalian expression vector for C terminal mScarlet-I fusionsDepositorTypeEmpty backboneTagsmScarlet-IExpressionMammalianAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH2B-miRFP670nano
Plasmid#127438PurposeHistone 2B labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertH2B-miRFP670nano (H2BC11 Synthetic, Human)
TagsmiRFP670nanoExpressionMammalianPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHis-hTG2
Plasmid#100719PurposeExpression of human transglutaminase 2 in E.coliDepositorInsertTGM2 (TGM2 Human)
TagsHisExpressionBacterialMutationsilent mutation Arg580 (AGG--CGT) to enhance expr…PromoterT7Available sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28-his-3C_Tau352
Plasmid#100092PurposeExpresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tagDepositorInsertFetal microtubule-associated protein tau (MAPT Human)
TagsHis6ExpressionBacterialMutationwild type sequencePromoterT7Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDicAID_nCas9-PmCDA_NptII_Della
Plasmid#91694PurposeDicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDellaDepositorInsertSpCas9
TagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9PromoterPcUbiAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-mFlagEts1
Plasmid#86099Purposeexpresses flag tagged Ets1 in mammalian cellsDepositorPublicationAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hFLT1
Plasmid#83435Purposehuman VEGFR1 with HA and FLAG tagDepositorInserthuman VEGFR1 (FLT1 Human)
TagsFlag and MycExpressionMammalianMutationHA-tag was inserted 30 AA downstream of the FLT1 …PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
eSrtA(2A-9) pET29b
Plasmid#75145Purposereprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificityDepositorInsertreprogrammed sortase A variant
Tags6x HisExpressionBacterialMutationS102C A104H E105D K138P K152I N160K K162H T164N K…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by