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Venus1-S727A-STAT3

Plasmid

#123174

Purpose

Expresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

S727A substitution

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-S727A-STAT3

Plasmid

#123174

Purpose

Expresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

S727A substitution

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-Y705F-STAT3

Plasmid

#123172

Purpose

Expresses Y705F STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

Y705F substitution

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-Y705F-STAT3

Plasmid

#123172

Purpose

Expresses Y705F STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

Y705F substitution

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-K685R-STAT3

Plasmid

#123170

Purpose

Expresses K658R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

K685R substitution

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-K685R-STAT3

Plasmid

#123170

Purpose

Expresses K658R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

K685R substitution

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-K49R-STAT3

Plasmid

#123166

Purpose

Expresses K49R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

K49R substitution

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-K140R-STAT3

Plasmid

#123168

Purpose

Expresses K140R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

K140R substitution

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-K140R-STAT3

Plasmid

#123168

Purpose

Expresses K140R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

K140R substitution

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-K49R-STAT3

Plasmid

#123166

Purpose

Expresses K49R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

K49R substitution

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-FKBP-Kras-Blasticidin

Plasmid

#120714

Purpose

Expresses membrane-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

amino acids 158-188

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-Kras-Blasticidin

Plasmid

#120714

Purpose

Expresses membrane-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

amino acids 158-188

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEFL GAL4dbd-KLF4 159-329

Plasmid

#140148

Purpose

GAL4-DNA binding domain fusion protein with amino acids 159 to 329 of human KLF4 (repressor domain)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 aa 159-329 (KLF4 Human)

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 1 to 158 and 330 to 479

Promoter

EF1a

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCEFL GAL4dbd-KLF4 159-329

Plasmid

#140148

Purpose

GAL4-DNA binding domain fusion protein with amino acids 159 to 329 of human KLF4 (repressor domain)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 aa 159-329 (KLF4 Human)

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 1 to 158 and 330 to 479

Promoter

EF1a

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTetOne-orangutan cGASdeltaN-HA

Plasmid

#186898

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Pongo abelii, Synthetic)

Use

Retroviral

Tags

HA

Mutation

N-terminal truncation

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTetOne-orangutan cGASdeltaN-HA

Plasmid

#186898

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Synthetic, Pongo abelii)

Use

Retroviral

Tags

HA

Mutation

N-terminal truncation

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE3G-orangutan cGASdeltaN-HA

Plasmid

#186872

Purpose

Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Pongo abelii, Synthetic)

Use

Retroviral

Tags

HA

Mutation

N-terminal truncation

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE3G-orangutan cGASdeltaN-HA

Plasmid

#186872

Purpose

Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Synthetic, Pongo abelii)

Use

Retroviral

Tags

HA

Mutation

N-terminal truncation

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE3G-orangutan cGASdeltaN-GFP

Plasmid

#186846

Purpose

Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal GFP tag (CGAS Pongo abelii, Synthetic)

Use

Retroviral

Tags

GFP

Mutation

N-terminal truncation

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE3G-orangutan cGASdeltaN-GFP

Plasmid

#186846

Purpose

Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal GFP tag (CGAS Synthetic, Pongo abelii)

Use

Retroviral

Tags

GFP

Mutation

N-terminal truncation

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge