We narrowed to 8,727 results for: pcDNA3

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pcDNA3-6XHis-human SUMO 2

Plasmid

#133771

Purpose

express 6Xhis- SUMO 2 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 2 (SUMO2 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3 91-710

Plasmid

#75140

Purpose

Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 91-710

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 TDP-43 NLS1 YFP

Plasmid

#84912

Purpose

Mammalian expresion of TDP-43 NLS1 YFP

Depositor

Aaron Gitler

Article

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Mutation

K82A, R83A, K84A

Promoter

CMV

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-10His-mStayGold (E138D)

Plasmid

#211363

Purpose

Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

mStayGold

Tags

10xHis-tag

Expression

Mammalian

Mutation

E138D

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.1-T2A-EGFP

Plasmid

#216489

Purpose

Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.1

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 1

Plasmid

#133770

Purpose

express 6Xhis- SUMO 1 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 1 (SUMO1 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6Xhis-human SUMO 3

Plasmid

#133772

Purpose

express 6Xhis- SUMO 3 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 3 (SUMO3 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-Tau2N4R-WPRE

Plasmid

#226381

Purpose

Expresses tau propagation ORF with V5 tag on WT 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-split-nLuc

Plasmid

#212612

Purpose

For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-split-nLuc

Use

Luciferase

Expression

Mammalian

Mutation

C373T mutation in CVB3

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 EYFP H148Q/I152L

Plasmid

#25872

Depositor

Peter Haggie

Article

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q/I152L

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with H148Q and I152L m…

Available Since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3 myc CIITA (P#808)

Plasmid

#14650

Depositor

Matija Peterlin

Article

Kanazawa et al Immunity. 2000 Jan . 12(1):61-70.

Insert

CIITA (CIITA Human)

Tags

Myc

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RLUC POLIRES FLUC

Plasmid

#45642

Purpose

Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

Depositor

Nahum Sonenberg

Article

Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

Insert

RLUC - IRES - FLUC

Use

Luciferase

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

Plasmid

#182287

Purpose

Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Inserts

codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT

Tags

nuclear export signal (NES)

Expression

Mammalian

Mutation

Y306A/Y384F (AF) double mutant of Methanosarcina …

Promoter

CMV and U6

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-E1799K

Plasmid

#69009

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-E1799K (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Glutamate 1799 to Lysine

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_miniSOG2 T2A H2B-EGFP

Plasmid

#87410

Purpose

Mammalian expression vector encoding Histone 2B GFP fusion and miniSOG2

Depositor

Xiaokun Shu

Article

Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

Insert

mini singlet oxygen generator 2

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-F1888L

Plasmid

#69010

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-F1888L (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Phenylalanine 1888 to Leucine

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only
1477 pcDNA3 flag HA Akt1

Plasmid

#9021

Depositor

William Sellers

Article

Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

Insert

Akt1 (AKT1 Human)

Tags

HA and flag

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ArchT-BFP2-TSERex

Plasmid

#123312

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

ArchT-BFP2

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-FP4-mito

Plasmid

#229692

Purpose

Transient expression of EGFP FP4-mito in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP; Mitochondira FP4 motifs from Listeria ActA

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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NC16 pCDNA3.1 FLAG NRF2

Plasmid

#36971

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

NRF2 (NFE2L2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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