We narrowed to 27,998 results for: STI
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Plasmid#140616PurposeExpression of OsTIR1F74A and mScarlet-mAID protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1 mutated F74A
ExpressionMammalianAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0782
Plasmid#133514PurposeExpression of sfGFP tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
ExpressionYeastAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C'5802
Plasmid#176879PurposeHigh constitutive expression of Firefly Luciferase in plantsDepositorInsertAtUbi10:Luc+:RbcsE9
UseLuciferaseAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-NP1
Plasmid#72654PurposeConstitutive expression of NP1DepositorAvailable SinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV0753
Plasmid#133487PurposeExpression in fission yeast from promoter pnmt81 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt81)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAID5XY
Plasmid#165051PurposeIntegrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSW614 - pREV
Plasmid#115989Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpREV
UseUnspecifiedAvailable SinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_ScPH1-S
Plasmid#131193PurposeExpresses ScPH1-S (Synechosystis PCC6803) preceded by restriction sitesDepositorInsertScPH1-S
ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPS0314
Plasmid#115507PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celBDepositorInsertcelB pOGG050, T-pharma pOGG003 assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP2283
Plasmid#70702PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialPromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSW618 - pAHP6
Plasmid#115991Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpAHP6
UseUnspecifiedAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW615 - pATHB-8
Plasmid#115990Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpATHB-8
UseUnspecifiedMutationA1287T to abolish Eco31I siteAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pconst #5-RFP
Plasmid#81079Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrPromoterConstitutive Promoter - BBa_J23110Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV0749
Plasmid#133483PurposeExpression in fission yeast from promoter ptdh1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(tdh1)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag 3A/WT myc-M1
Plasmid#87719PurposeExpresses WT myc-M1 spastin isoform in mammalian cellsDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-CMV-CGFP-mAID
Plasmid#140606PurposeExpression of OsTIR1 and mAID-GFP protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1
ExpressionMammalianAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW609 - N001
Plasmid#115993Purposeintermediate vector for GreenGate cloning method, contains modules from A-FDepositorInsertH-A adapter:pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseUnspecifiedTagsHDEL and SP(ER)Available SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-puro-CLIP-FLAG-CD59
Plasmid#50375PurposeExpress CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cellsDepositorInsertCLIP
ExpressionMammalianPromoterSR alphaAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV0758
Plasmid#133511PurposeExpression of 3GFP tagged RasAct probe to monitor active Ras1DepositorInsertpLys3(BstZ17I)-p(pak1)-RasAct(3xbyr2RBD)-3GFP-kanMX-terminator(ScADH1)
ExpressionYeastAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only