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pdnCas3-CDA1

Plasmid

#229535

Purpose

pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

Depositor

Kevin Verstrepen

Article

Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

Inserts

dnCas3
Cytidine deaminase
Uracil glycosylase inhibitor

Tags

XTEN

Expression

Bacterial and Yeast

Mutation

codon optimized for S.cerevisiae and codon optimi…

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pdnCas3-APOBEC1

Plasmid

#229537

Purpose

pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

Depositor

Kevin Verstrepen

Article

Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

Inserts

rAPOBEC1
dnCas3
Uracil glycosylase inhibitor

Tags

XTEN

Expression

Bacterial and Yeast

Mutation

codon optimized for S.cerevisiae and codon optimi…

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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ECFP-FRB-Sec61b

Plasmid

#210290

Purpose

nucleus OM marker and "anchor" for FKBP-FRB CID

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

ECFP-FRB-Sec61b

Tags

ECFP and FRB

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Tom20-EYFP-miLID

Plasmid

#210291

Purpose

mitochondria marker and "anchor" for optogenetics

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

Tom20-EYFP-miLID

Tags

EYFP and iLID

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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mActA-mSspB-mCherry

Plasmid

#210294

Purpose

ActuAtor for iLID optogenetics

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

mActA-,SspB-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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ECFP-FRB-GiantinS

Plasmid

#210296

Purpose

Golgi marker and anchor for FKBP-FRB CID

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

ECFP-FRB-GiantinS

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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EYFP-FKBP-DN-N-WASP

Plasmid

#210301

Purpose

control for targeted actin nucleation

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

EYFP-FKBP-DN-N-WASP

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2h)3(S4h)3-gs-P6f

Plasmid

#228083

Purpose

Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers, N-term TagBFP, and C-term P6f; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2h, S4h, P6f

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB32-CrActin-35S

Plasmid

#226708

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from CaMV 35S promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrActin

Use

CRISPR

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB32-CrActin-UBI

Plasmid

#226709

Purpose

For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

Depositor

Fenglin Deng

Article

Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

Insert

CrActin

Use

CRISPR

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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RNF149 shRNA-TRE

Plasmid

#225337

Purpose

shRNAmir backbone under tet-operator for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF149 shRNA (Rnf149 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

TRE (tetracycline-responsive element)

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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USP7 shRNA-TRE

Plasmid

#225338

Purpose

shRNAmir backbone under tet-operator for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

USP7 shRNA (Usp7 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

TRE (tetracycline-responsive element)

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Luciferase shRNA-TRE

Plasmid

#225339

Purpose

shRNAmir backbone under tet-operator for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

Luciferase shRNA (LOC116160065 Mouse)

Use

AAV and RNAi

Expression

Mammalian

Promoter

TRE (tetracycline-responsive element)

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B2-HA

Plasmid

#209227

Purpose

Mammalian expression of ATP8B2

Depositor

Hye-Won Shin

Article

Takatsu et al Mol Cell Biol. 2024 Sep 2:1-16. doi: 10.

Insert

ATP8B2 (ATP8B2 Human)

Use

Retroviral

Tags

HA

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-IRAK4-mScarlet-P2A-Puro

Plasmid

#221237

Purpose

Stable expression of mouse IRAK4-mScarlet after Lentiviral transduction

Depositor

Jonathan Kagan

Article

Fisch et al Nature. 2024 Jul;631(8021):635-644. doi:

Insert

IRAK4 (Irak4 Mouse)

Use

Lentiviral

Tags

mScarlet

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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plenti hSyn AldoC

Plasmid

#220917

Purpose

Neuronal specific expression of AldoC

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

AldoC (ALDOC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hSyn

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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CMV DJ-1 C106A

Plasmid

#220914

Purpose

Expresses DJ-1 with the C106A mutation

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

DJ-1 (PARK7 Human)

Expression

Mammalian

Mutation

C106A

Promoter

CMV

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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NL1

Plasmid

#218404

Purpose

NL1 insert in AAV9 capsid

Depositor

Casey Maguire

Article

Hanlon et al Molecular Therapy. 2024 Jun 5. doi.org/1

Insert

NL1 7mer (LTTEGRR)

Use

AAV

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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TH1

Plasmid

#218405

Purpose

TH1 insert in AAV9 capsid

Depositor

Casey Maguire

Article

Hanlon et al Molecular Therapy. 2024 Jun 5. doi.org/1

Insert

TH1 7mer (HPARALP)

Use

AAV

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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OR5-consensus

Plasmid

#208062

Purpose

Consensus OR5 engineered from human OR5 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR5-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR51-consensus

Plasmid

#208065

Purpose

Consensus OR51 engineered from human OR51 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR51-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR6-consensus

Plasmid

#208063

Purpose

Consensus OR6 engineered from human OR6 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR6-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR8-consensus

Plasmid

#208064

Purpose

Consensus OR8 engineered from human OR8 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR8-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR1-consensus

Plasmid

#208059

Purpose

Consensus OR1 engineered from human OR1 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR1-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR2-consensus

Plasmid

#208060

Purpose

Consensus OR2 engineered from human OR2 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR2-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR10-consensus

Plasmid

#208058

Purpose

Consensus OR10 engineered from human OR10 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR10-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR4-consensus

Plasmid

#208061

Purpose

Consensus OR4 engineered from human OR4 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR4-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OR52-consensus

Plasmid

#208080

Purpose

Consensus OR52 engineered from human OR52 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR52-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-mCh-GATA2-R398W

Plasmid

#193084

Purpose

constitutive expression of mCherry fused to human Gata2 with mutation R398W in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-GATA2-R398W

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-Gata2-C373R

Plasmid

#193085

Purpose

constitutive expression of mouse Gata2 with mutation C373R in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

Gata2-C373R

Use

Lentiviral

Expression

Mammalian

Promoter

Ubc

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-Gata2-L359V

Plasmid

#193086

Purpose

constitutive expression of mouse Gata2 with mutation L359V in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

Gata2-L359V

Use

Lentiviral

Expression

Mammalian

Promoter

Ubc

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-MD-Gata2

Plasmid

#193090

Purpose

constitutive expression of the mitosis degradation (MD) domain of cyclin B1 fused to mouse Gata2 in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

MD-Gata2

Use

Lentiviral

Expression

Mammalian

Promoter

Ubc

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-MD-mTurquoise

Plasmid

#193094

Purpose

constitutive expression of the mitosis degradation (MD) domain of cyclin B1 fused to mTurquoise in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

MD-mTurquoise

Use

Lentiviral

Expression

Mammalian

Promoter

Ubc

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-mCh-GATA2

Plasmid

#193071

Purpose

constitutive expression of mCherry fused to human Gata2 in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-GATA2

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-mCh-GATA2-R293Q

Plasmid

#193072

Purpose

constitutive expression of mCherry fused to human Gata2 with mutation R293Q in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-GATA2-R293Q

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-mCh-GATA2-P304H

Plasmid

#193073

Purpose

constitutive expression of mCherry fused to human Gata2 with mutation P304H in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-GATA2-P304H

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-mCh-GATA2-R307W

Plasmid

#193074

Purpose

constitutive expression of mCherry fused to human Gata2 with mutation R307W in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-GATA2-R307W

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-mCh-GATA2-A318T

Plasmid

#193075

Purpose

constitutive expression of mCherry fused to human Gata2 with mutation A318T in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-GATA2-A318T

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-mCh-GATA2-L321F

Plasmid

#193076

Purpose

constitutive expression of mCherry fused to human Gata2 with mutation L321F in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-GATA2-L321F

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-mCh-GATA2-R330Q

Plasmid

#193077

Purpose

constitutive expression of mCherry fused to human Gata2 with mutation R330Q in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-GATA2-R330Q

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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