We narrowed to 152,678 results for: des

1. LENTICRISPR-UPP1_sgRNA1

   Plasmid

   #201634

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   UPP1 (UPP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CPSF5

   Plasmid

   #155480

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   CPSF5 (NUDT21 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LENTICRISPR-UPP1_sgRNA2

   Plasmid

   #201635

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   UPP1 (UPP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLgw V5-EcoDam

   Plasmid

   #59210

   Purpose

   Mammalian DamID control lentiviral vector for expression of V5-Dam only

   Depositor

   Bas van Steensel

   Article

   Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

   Insert

   EcoDam
   

   Use

   Lentiviral; Damid

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter and heat shock minima…

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. non-targeting control gRNA (BRDN0001149198)

   Plasmid

   #80248

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEntry_MERS-CoV-Spike

   Plasmid

   #168837

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   MERS-CoV-Spike (S )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBabe_Hyg_cOVA_T2A_mStrawberry

   Plasmid

   #161737

   Purpose

   Expression of cOVA and mStrawberry

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   cytoplasmic ovalbumin
   

   Use

   Retroviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCas9-CtIP

   Plasmid

   #109403

   Purpose

   Expresses Cas9 fusion to human CtIP protein

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   CtIP (RBBP8 Human)
   

   Use

   Tags

   Expression

   Mutation

   full length CtIP

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HRAS

   Plasmid

   #55653

   Purpose

   expression of mouse HRAS

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   HRAS (Hras Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCas9-HE-Geminin

    Plasmid

    #109402

    Purpose

    Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    HE-Geminin
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SPHK2 gRNA (BRDN0001148465)

    Plasmid

    #77093

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PRKCE gRNA (BRDN0001148049)

    Plasmid

    #76791

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCE

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCE (PRKCE Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SPHK1 gRNA (BRDN0001147454)

    Plasmid

    #76813

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. non-targeting control gRNA (BRDN0001145694)

    Plasmid

    #80191

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-Ub KO

    Plasmid

    #11934

    Depositor

    Nico Dantuma

    Article

    Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

    Insert

    Ubiquitin KO (RBX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    All 7 lysines of ubiquitin mutated to arginines.

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

    Plasmid

    #174515

    Purpose

    Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Inserts

    Mm PylS
    MmtRNA-Pyl-opt(UGA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ASL from the serT gene (UGA anticodon) and recoded

    Promoter

    GlnS and lpp

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBABE-hygro-BAP1-HA

    Plasmid

    #154020

    Purpose

    Mammalian Expression of HA-tagged Wild-Type BAP1

    Depositor

    Samuel Peña-Llopis

    Article

    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 6691 Bicistronic_ires_puro

    Plasmid

    #64335

    Purpose

    This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. non-targeting control gRNA (BRDN0001148862)

    Plasmid

    #80263

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RNF130 shRNA-TRE

    Plasmid

    #225336

    Purpose

    shRNAmir backbone under tet-operator for RNA interference, with YFP

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    RNF130 shRNA (Rnf130 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (tetracycline-responsive element)

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pENTR-AIF

    Plasmid

    #16182

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    Apoptosis-inducing factor (AIFM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSC5GGv1

    Plasmid

    #194645

    Purpose

    pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. V2R-GFP

    Plasmid

    #208910

    Purpose

    encodes the human vasopressin receptor 2, tagged with GFP

    Depositor

    Christian Gruber

    Article

    Di Giglio et al Sci Rep. 2017 Feb 1;7:41002. doi: 10.103

    Insert

    AVPR2 (AVPR2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pINDUCER20_mStrawberry

    Plasmid

    #161734

    Purpose

    Doxycycline-inducible expression of mStrawberry

    Depositor

    Alec Kimmelman

    Article

    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAcGP67A-TfR

    Plasmid

    #12130

    Depositor

    Pamela Bjorkman

    Article

    Lebron et al Cell. 1998 Apr 3. 93(1):111-23.

    Insert

    transferrin receptor (TFRC Human)
    

    Use

    Tags

    6X-His tag, Factor Xa cleavage site, and leader p…

    Expression

    Insect

    Mutation

    The portion of the human TfR gene encoding residu…

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

26. pGLOW30

    Plasmid

    #172697

    Purpose

    C. elegans mScarlet co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-2::mScarlet
    

    Use

    Tags

    mScarlet

    Expression

    Worm

    Mutation

    Promoter

    myo-2

    Available since

    Sept. 2, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. PT3.5/CMV-EGFRvIII

    Plasmid

    #20280

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    EGFRvII (EGFR Human)
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    See depositor comments below.

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TAPBPL pmRFP-N2 (721)

    Plasmid

    #62000

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    TAPBPL (TAPBPL Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pKLV-EF1amCherry-2A-OsTIR1-W

    Plasmid

    #159296

    Purpose

    Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    OsTIR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1a promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EYFP-p65

    Plasmid

    #111192

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

    Insert

    RELA (RELA Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PABPC1

    Plasmid

    #155805

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PABPC1 (PABPC1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEntry_MERS-CoV-nsp3

    Plasmid

    #168838

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    MERS-CoV-nsp3 (orf1ab )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-CMV-N-HA-Pcdh15_mini_V4

    Plasmid

    #199185

    Purpose

    An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

    Depositor

    David Corey

    Article

    Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

    Insert

    HA-mmPcdh15_mini_V4-CD2
    

    Use

    AAV

    Tags

    Human influenza hemagglutinin (HA)

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-CAG-FLuc

    Plasmid

    #83281

    Purpose

    recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

    Depositor

    Mark Kay

    Article

    Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

    Insert

    Firefly Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEntry_HCoV-OC43-Hemagglutinin-esterase

    Plasmid

    #168943

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-OC43-Hemagglutinin-esterase (HE )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TVBB C-term-mNeongreen-Blast

    Plasmid

    #169229

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen-P2A-Blast
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. YCplac33-NAT_MCART1

    Plasmid

    #140591

    Purpose

    Express MCART1 in S. cerevisiae

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    SLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    codon-optimized for expression in S. cerevisiae

    Promoter

    NDT1 promoter and 3'UTR

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA

    Plasmid

    #109314

    Purpose

    Plasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNA

    Depositor

    William Lagor

    Article

    Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LacZ in pLX304

    Plasmid

    #42560

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    2xV5

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCLXEBR-pTF-cKit

    Plasmid

    #114293

    Purpose

    TET-inducible expression of c-Kit

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    cKit (Kit Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge