We narrowed to 28,443 results for: Tat
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Plasmid#206979PurposeExpresses SauriABE8e by EFS promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoterDepositorInsertLmna sgRNA
UseAAV; Adenine base editorPromoterU6Available SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-MGT4-d2EGFP_CMV-mCherry
Plasmid#213764PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertMGT#4-d2EGFP
UseSynthetic BiologyAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-CLGT3-d2EGFP_CMV-mCherry
Plasmid#213766PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertCLGT#3-d2EGFP
UseSynthetic BiologyAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMuLE (L1-L4) 6X PRE
Plasmid#206168PurposepMuLE L1-L4 entry plasmid containing 6 consensus PGR bindings sites upstream of a minimal 2YBTATA promoterDepositorInsert6X PRE
UseGateway cloning entry clonePromoter2YBTATAAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMuLE (L1-L4) 2X PRE
Plasmid#206166PurposepMuLE L1-L4 entry plasmid containing 2 consensus PGR bindings sites upstream of a minimal 2YBTATA promoterDepositorInsert2X PRE
UseGateway cloning entry clonePromoter2YBTATAAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2/sh-FYN1-GFP_Seq_C
Plasmid#201399PurposeKnockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertFYN
ExpressionMammalianAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB_ROSA26_SpCas9_gRNAs
Plasmid#174703PurposePlasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.DepositorInsertSpCas9 dual gRNAs
UseAAVAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
DDX41_p.L237F_p.P238T_EGFP
Plasmid#175845PurposeExpresses DDX41 with mutationDepositorInsertDDX41 (DDX41 Human)
TagsEGFPExpressionMammalianMutationc.711G>T, p.L237F and c.712C>A, p.P238TPromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-GgVCL/*TBM
Plasmid#162790PurposeMUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cellsDepositorArticleInsertMutated Gallus gallus vinculin (G454V) (VCL Chicken)
TagsIRES-GFPExpressionBacterial and MammalianMutationchanged Gly 454 to ValPromoterbeta actinAvailable SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔH1 Δ5H
Plasmid#163582Purposeexpress Dcp2 ΔH1 Δ5H at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
TagsGFPExpressionYeastMutationL255A; mutate 440-450, 489-500, 701-708, 827-831,…PromoterDCP2 promoterAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2C Δ5H
Plasmid#163581Purposeexpress Dcp2C Δ5H at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
TagsGFPExpressionYeastMutationTruncation of aa1-300; mutate aa440-450, aa489-50…PromoterDCP2 promoterAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGAmut
Plasmid#153548PurposeUsed as a donor vector for the targeted knock-in of a mutation in PIGA exon 6DepositorInsertmutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
UseDonor plasmidTagsN/AMutationa 3-bp mutation in exon 6PromoterNo promoterAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT215
Plasmid#134818PurposeSMT205 with DHFR-FLAG-tag, western blotDepositorInsertsDihydrofolate reductase
Thymidylate synthase
TagsFLAGExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT101
Plasmid#134815PurposeDual expression of DHFR and TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7Available SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS314-U6-G96C/A97C/U100C/U101C
Plasmid#82328PurposeU6 telestem mutant (G96C/A97C/U100C/U101C) for in vivo studiesDepositorInsertU6 RNA with G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
ExpressionYeastMutationU6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS314-U6-A34G/A35G/G96C/A97C
Plasmid#81238PurposeU6 telestem mutant (A34G/A35G/G96C/A97C) for in vivo studiesDepositorInsertU6 RNA with A34G/A35G/G96C/A97C mutation (snR6 Budding Yeast)
ExpressionYeastMutationU6 telestem mutant (A34G/A35G/G96C/A97C)Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT201
Plasmid#134816PurposeSMT101 with TET promoter for TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSiren_EGFP-shHmga2#2
Plasmid#122290PurposeKnockdown for mouse Hmga2DepositorAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m7
Plasmid#84726PurposepVR2 but with a G to U mutation immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutationG to U mutation at position 41 of annotated "…Available SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m8
Plasmid#84727PurposepVR2 but with two G to U mutations immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutationG to U mutations at positions 40 and 41 of annota…Available SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only