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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,445 results for: grn

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Showing: 2861 - 2880 of 15445 results

  15. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid
    #136659
    Purpose
    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136659-plasmid-map-sequence-id-273359

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  16. sgRNA EMX1

    Plasmid
    #128121
    Purpose
    Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)
    Depositor
    Dominik Niopek
    Publication
    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA EMX1 + GFP
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    RSV/U6
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    128121-plasmid-map-sequence-id-249653

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  17. pRP_8xgRNA-FLuc

    Plasmid
    #135937
    Purpose
    Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.
    Depositor
    Antonio Amelio
    Publication
    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    minCMV
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    135937-plasmid-map-sequence-id-271493

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Showing: 2861 - 2880 of 15445 results