We narrowed to 16,641 results for: Pol;

1. pRSFDuet-1(nsp8-nsp7)(nsp12)

   Plasmid

   #165451

   Purpose

   Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

   Depositor

   Marc Delarue

   Article

   Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

   Inserts

   nsp12
   nsp8
   nsp7
   

   Tags

   14Histidine-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINE GFP nanobody:Halo:His6

   Plasmid

   #111090

   Purpose

   Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

   Depositor

   Lennart Wirthmueller

   Article

   Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

   Insert

   GFP Nanobody
   

   Tags

   Halo and His6

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   T7 promoter

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C1-AR Q48

   Plasmid

   #86428

   Purpose

   Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

   Depositor

   Michael Mancini

   Article

   Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

   Insert

   Androgen receptor (AR) (AR Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Poly-glutamine expansion, G130D

   Promoter

   CMV

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1-AR Q0

   Plasmid

   #86427

   Purpose

   Fluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine region

   Depositor

   Michael Mancini

   Article

   Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

   Insert

   androgen receptor (AR) (AR Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of the poly-glutamine expansion

   Promoter

   CMV

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti HsATP13A3 WT

   Plasmid

   #195849

   Purpose

   Expresses wild-type Homo sapiens ATP13A3

   Depositor

   Veerle Baekelandt

   Article

   Houdou et al Biomolecules. 2023 Feb 9;13(2):337. doi:

   Insert

   ATPase 13A3 (ATP13A3 Human)
   

   Use

   Lentiviral

   Mutation

   D498N, L956P, M850I, V855M, R858H, L675V

   Promoter

   CMV

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-PARP1-3xFlag-7xKQ

   Plasmid

   #111576

   Purpose

   Expresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   PARP1 (PARP1 Human)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   lysine to glutamine mutation at the following ami…

   Promoter

   CMV

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEcRNAP6

   Plasmid

   #128940

   Purpose

   pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z

   Depositor

   Seth Darst

   Article

   Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

   Inserts

   RpoA (rpoA E. coli)
   RpoB
   RpoC-ppx(His)10
   RpoZ
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   PreScission protease site (GE Healthcare; LEVLFQG…

   Promoter

   T7

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFRT-TO-RPB1-His WT CRres si2,4R

   Plasmid

   #139404

   Purpose

   Dox-inducible, wt C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

   Depositor

   Jesper Svejstrup

   Article

   Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

   Insert

   RPB1 (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   pCMV, TRex

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEXP5-aHL_spytag_his

   Plasmid

   #107334

   Purpose

   Monomeric SpyTag-6×-His alpha-hemolysin

   Depositor

   George Church

   Article

   Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   alpha-hemolysin-SpyTag-6xHis
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFRT-TO-RPB1-His K1268R CRres si2,4R

    Plasmid

    #139405

    Purpose

    Dox-inducible, K1268R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    K1268R

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS2230

    Plasmid

    #158420

    Purpose

    AAV plasmid with Ple253 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple253 (PITX3 )
    

    Use

    AAV

    Promoter

    Ple253

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. RRL.sin.cPPT.CMV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG494)

    Plasmid

    #139447

    Purpose

    Lentiviral vector to ectopically express human NCOA7 isoform 4 from CMV promoter

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. RRL.sin.cPPT.SFFV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG536)

    Plasmid

    #139443

    Purpose

    Lentiviral vector to ectopically express human NCOA7 isoform 4 from SFFV promoter

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRT-TO-RPB1-His K1278R CRres si2,4R

    Plasmid

    #139406

    Purpose

    Dox-inducible, K1278R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    K1278R

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiGuide-Neo-NCOA7g1 (BB34)

    Plasmid

    #139452

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT-TO-RPB1-His CRres K619,627,642R si2,4R

    Plasmid

    #139409

    Purpose

    Dox-inducible, K619, 627, 642R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    K619, 627, 642R

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFRT-TO-RPB1-His K751,758,767R CRres si2,4R

    Plasmid

    #139408

    Purpose

    Dox-inducible, K751, 758, 767R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    K751, 758, 767R

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFRT-TO-RPB1-His K710,719R CRres si2,4R

    Plasmid

    #139407

    Purpose

    Dox-inducible, K710, 719R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    K710,719R

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pspCas9-3exo-Tetra-com-CLCN5-sp-g1

    Plasmid

    #176236

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

    Plasmid

    #176237

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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