We narrowed to 5,647 results for: donor

Showing: 2881 - 2900 of 5647 results

Results per page:

Sort results by:

KHBD00016

Plasmid

#34178

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8361 (E(spl)m7-HLH Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00024

Plasmid

#34186

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1007 (emc Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRc2_BirA_NRF2

Plasmid

#136524

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2 (NFE2L2 Human)

Use

Entry vector for gateway cloning

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCM-AAVS1-TO-hNGN2

Plasmid

#105840

Purpose

Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
mCherry
rtTA3G

Use

CRISPR and TALEN

Promoter

CAG, EF-1alpha, and TRE3G

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Puro_siKD

Plasmid

#86695

Purpose

Tetracycline inducible expression of shRNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Expression

Mammalian

Promoter

H1 TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Tubb3-GFP KI

Plasmid

#131497

Purpose

Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Tubb3, Tubb3 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-FluoSTEP-AKAR

Plasmid

#181844

Purpose

Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

FluoSTEP-AKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

Plasmid

#89991

Purpose

Donor template for generation of SOX2-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)

Use

CRISPR

Promoter

none

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-empty-DSE-TetO-8kb

Plasmid

#213022

Purpose

piggyBac donor vector containing an 8-kb TetO operator array for random integration using PBase transposase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

TetO

Use

Mouse Targeting; Piggybase integration (transposo…

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pC13N-iCAG.copGFP

Plasmid

#66578

Purpose

CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

copGFP

Expression

Mammalian

Promoter

CAG

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
[#GS24 -LV] Syn1-GFP-OMP25

Plasmid

#233566

Purpose

Lentiviral construct of mitochondrial localized GFP to be expressed in the donor cells under the Synapsin1 promotor

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

eGFP (eGFP Synthetic, Aequorea victoria)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
HR180-LGR5-iCT

Plasmid

#129094

Purpose

KI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

iCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1P-iCAG.copGFP

Plasmid

#66577

Purpose

AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-AAVS1 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

copGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

Plasmid

#89992

Purpose

Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

Insert

OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)

Use

CRISPR

Promoter

none

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSA127 A2UCOE-Cas9-BFP

Plasmid

#249151

Purpose

Overexpression of SpCas9-BFP and blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

A2UCOE-Cas9-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00202

Plasmid

#34353

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG33261 (Trl Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pUFlip-floxed2A-mRFP-; gcry1:BFP -2

Plasmid

#173888

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pNKX2-5-T2A-GFP-PGK-Puro

Plasmid

#203607

Purpose

Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

Depositor

Matthew Birket

Article

Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

Insert

NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)

Use

Human targeting

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-PDL1

Plasmid

#159280

Purpose

Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Insert

Human PDL1/CD274 (CD274 Human)

Use

AAV; S1 knockin donor vector

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-Tet-OsTIR1(F74G)-V5

Plasmid

#158664

Purpose

Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

Tet-OsTIR1(F74G) AAVS1

Use

CRISPR

Expression

Mammalian

Mutation

F74G

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge