We narrowed to 3,931 results for: SPL
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Plasmid#226556PurposeMinigene encoding the CEP290 c.2991+1655A>G mutationDepositorInsertCEP290 (CEP290 Human)
UseTags3xFLAGExpressionMammalianMutationc.2991+1655A>G intronic mutationPromoterAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-SUMO-cSrc-SH2-AviTag
Plasmid#214230PurposeBacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selectionsDepositorInsertcSrc kinase SH2 domain (SRC Human)
UseTags6xHis, AviTag, and SUMOExpressionBacterialMutationPromoterT7Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTR-UF11
Plasmid#157970PurposeAAV vector plasmid containing the Chimeric CMV/Chicken Beta Actin promoter driving 'humanized' GFPDepositorHas ServiceAAV.A01.T, AAV.A02.T, AAV.A03.T, AAV.A04.T, AAV.A05.T, AAV.A06.T, AAV.A07.T, AAV.A08.T, AAV.A09.T, AAV.A10.T, AAV.A11.T, and AAV.A12.TInsert'humanized' e.g. codon optimized GFP
UseAAVTagsExpressionMutationPromoterchimeric CMV/Chicken Beta actin (CBA)Available sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL452(hygro)-Sf3b1-K700K
Plasmid#90426Purposeselectable HDR vector to introduce silent mutation (position 700) in mus Sf3b1 gene (for use with pL452-Sf3b1-K700E and sgSf3b1(T1) gRNA vectors enabling generation of hemizygous K700E mutation)DepositorInsertSf3b1 - left and right homology arms (Sf3b1 Mouse)
UseHomology-directed repair vectorTagsExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXPR_sgFLNB-SK2
Plasmid#119873PurposegRNA expression vectorDepositorInsertsgFLNB-SK2 (FLNB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXPR_sgFLNB-SK4
Plasmid#119874PurposegRNA expression vectorDepositorInsertsgFLNB-SK4 (FLNB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bad-4E-pEGFP-C1
Plasmid#166740PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation in BH3 domain to disrupt binding anti-apoptotic proteinsDepositorInsertBad-4E (BAD Human)
UseTagsVenusExpressionMammalianMutation4 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-sgCD20
Plasmid#209749PurposeLentiviral transfer plasmid to express Cas9 and a gRNA targeting the human CD20 gene, MS4A1.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-NeoR_dCas9-Sniper-KRAB_sgSTK3i_#2 (NeoR)
Plasmid#209775PurposeCRISPRi for STK3DepositorInsertdCas9-Sniper-KRAB, sgSTK3i (STK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB-6HIS-TEV-mEGFP,IRES-NGFR
Plasmid#164521PurposeAll-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC23A1_STOP
Plasmid#161179PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC23A1 (SLC23A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABE (hygro) mEOS2 hYAP 1-2delta
Plasmid#124162PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertmEOS2-YAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hE2F7mg_115mut
Plasmid#73077Purposehuman E2F7 minigene (exons 11,12,13/introns cassette) with inserted SNORD115 binding siteDepositorInsertE2F7 (E2F7 Human)
UseTagsExpressionMammalianMutationpartial gene, exons 11,12,13 spaced by introns, m…PromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRP.ABCA4 c.5461-10T
Plasmid#226553PurposeMinigene encoding the ABCA4 c.5461-10T mutationDepositorInsertABCA4 (ABCA4 Human)
UseTagsExpressionMammalianMutationc.5461-10T intronic mutationPromoterCAGAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRP.ATM c.7865C>T
Plasmid#226554PurposeMinigene encoding the ATM c.7865C>T mutationDepositorInsertATM (ATM Human)
UseTags3xFLAGExpressionMammalianMutationc.7865C>TPromoterAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDH-V1-CD20-Puro
Plasmid#209755PurposeLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 1 mRNA isoform.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-V2-CD20-Puro
Plasmid#209756PurposeLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 2 mRNA isoform.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC23A3_STOP
Plasmid#161203PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC23A3 (SLC23A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET3d-SF-DCTN1-TS
Plasmid#169375PurposeE. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.DepositorInsertdynactin subunit 1 isoform 1 (DCTN1 Human)
UseTags6xHis Tag, SplitFlAsH Tag, and TwinStrep TagExpressionBacterialMutationPoint mutations: A139T, I304T, Q322R, T516A, E577…PromoterAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bad-2A-pEGFP-C1
Plasmid#166748PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation to 2 positions in the BH3 domainDepositorInsertBad-2A (BAD Human)
UseTagsVenusExpressionMammalianMutation2 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only