We narrowed to 8,451 results for: Set;

1. pAS_4xMma PylT_FLAG-Mma PylRS AF

   Plasmid

   #140023

   Purpose

   Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y271A, Y349F

   Promoter

   EF1

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PLJR962

   Plasmid

   #115162

   Purpose

   Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

   Depositor

   Sarah Fortune

   Article

   Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

   Inserts

   Sth1 sgRNA scaffold
   Sth1 dCas9
   TetR
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PLJR965

   Plasmid

   #115163

   Purpose

   Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

   Depositor

   Sarah Fortune

   Article

   Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

   Inserts

   Sth1 sgRNA scaffold
   Sth1 dCas9
   TetR (deleted TetON) MtbCO
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS_4xMma PylT_GFP 150TAG 6xHis

   Plasmid

   #140015

   Purpose

   Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   150TAG

   Promoter

   EF1

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLM-CMV-R-Cre

   Plasmid

   #27546

   Purpose

   Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

   Depositor

   Michel Sadelain

   Article

   Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

   Insert

   mCherry_P2A_Cre recombinase
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TAC-OK+9MS-Cas9

   Plasmid

   #120354

   Purpose

   piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OK+9MS cassette
   

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Klf4 [1-483]

   Promoter

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCasso

   Plasmid

   #207530

   Purpose

   Conditionally-replicating in Pseudomonas plasmid for cytidine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sg

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   cytidine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgRNA
   

   Use

   CRISPR; Pseudomonas vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV hSyn-fDIO-taCasp3-TEVp

   Plasmid

   #207997

   Purpose

   Inducing apoptotic ablation of neurons through a process reliant on Flp recombinase

   Depositor

   Kazunari Miyamichi

   Article

   Goto et al Neuron. 2025 Feb 17:S0896-6273(25)00041-

   Insert

   taCasp3-TEVp
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.10/RSV_SF3B3-miniXon_Luciferase

   Plasmid

   #174660

   Purpose

   Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.

   Depositor

   Beverly L. Davidson

   Article

   Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

   Insert

   miniXon cassette
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Kozak ATG initiation sequence in middle exon

   Promoter

   RSV

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAblo

    Plasmid

    #207528

    Purpose

    Conditionally-replicating in Pseudomonas plasmid for adenine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→ ABE:SpRY, PEM7→non-specific sg

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→ ABE:SpRY, PEM7→non-specific sgRNA
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_B2303

    Plasmid

    #91068

    Purpose

    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-hSyn-nucGFP

    Plasmid

    #140190

    Purpose

    Overexpression of GFP with a nuclear localization sequence, under the human synapsin promoter

    Depositor

    Lorenz Studer

    Article

    Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

    Insert

    GFP-NLS
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS_4xMx PylT_GFP 150TAG

    Plasmid

    #140016

    Purpose

    Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG, K46E (Please see depositor comments below)

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. MP9956:SFG.GD3synthase-2A-GD2synthase

    Plasmid

    #75013

    Purpose

    Retroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.

    Depositor

    Martin Pule

    Article

    Thomas et al PLoS One. 2016 Mar 31;11(3):e0152196. do

    Inserts

    murine GD3 synthase
    murine GD2 synthase
    

    Use

    Retroviral; Momlv retroviral vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSS146

    Plasmid

    #64770

    Purpose

    For integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminator

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    Cre-EBD78
    

    Use

    Tags

    fused to the human Estrogen Binding Domain (EBD),…

    Expression

    Yeast

    Mutation

    Promoter

    TDH4

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_4xMma PylT_FLAG-Mma PylRS

    Plasmid

    #140009

    Purpose

    Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVTRE3_tdTomato

    Plasmid

    #135178

    Purpose

    AAV expression of TRE(TRE3G)-driven, tdTomato expression for fluorescent labling

    Depositor

    Tetsuo Yamamori

    Article

    Abe et al Front Neuroanat. 2018 Oct 30;12:89. doi:

    Inserts

    tdTomato
    TRE3G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    a variant of DsRed

    Promoter

    TRE3G

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVS1-Puro-CAG-mNG2(1-10)

    Plasmid

    #206042

    Purpose

    Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

    Insert

    AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
    

    Use

    CRISPR and Synthetic Biology; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL4.10/RSV_SF3B3-Xon_Luciferase

    Plasmid

    #174659

    Purpose

    Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.

    Depositor

    Beverly L. Davidson

    Article

    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

    Insert

    Xon cassette
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Kozak ATG initiation sequence in middle exon

    Promoter

    RSV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAluYa5-neo-TET

    Plasmid

    #51283

    Purpose

    expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    AluYa5
    

    Use

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Mutation

    Promoter

    upstream pol III enhancer sequence of the 7SL gen…

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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