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pMSCV-MAVS-M1A,M142A(KaganE20)

Plasmid

#52011

Purpose

Expresses the human MAVS CDS containing the point mutations M1A and M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A,M142A

Use

Retroviral

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pFUS2_b3

Plasmid

#50697

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pFUS2_b2

Plasmid

#50696

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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p3HD

Plasmid

#50672

Purpose

Contains third TALE repeat harboring HD as RVD

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

repeat 3HD

Use

TALEN

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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γA3/N-GFP

Plasmid

#33113

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Cytoplasmic domain has been replaced with the N-c…

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
Δ741-752Δconst-GFP

Plasmid

#33120

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of aa 741-752 along with the entire cons…

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pET-sCS4T

Plasmid

#28249

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

single chain Cse4-Scm3-H4 with Thrombin site

Tags

His

Expression

Bacterial

Mutation

A thrombin cutting site between Cse4 and Scm3

Available Since

July 12, 2011

Availability

Academic Institutions and Nonprofits only
pET-sCS4T+N

Plasmid

#28255

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

sCS4T+N

Tags

His

Expression

Bacterial

Mutation

single chain CSE4-SCM3-H4 with Thrombin site, SCM…

Available Since

July 12, 2011

Availability

Academic Institutions and Nonprofits only
pET-sC4T

Plasmid

#28251

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

single chain Cse4-H4 with Thrombin site

Tags

His

Expression

Bacterial

Mutation

A thrombin cutting site between Cse4 and H4

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pV1338

Plasmid

#111443

Purpose

Solo vector pV1326 + sgScADE2

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgScADE2

Use

CRISPR

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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p21-Reporter

Plasmid

#52432

Purpose

DNA damage inducible Red Fluorescent Protein for mammalian expression

Depositor

Joel Voldman

Article

Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

Insert

p21-p53 Tandem Response Element Promoter

Tags

TurboRFP

Expression

Mammalian

Promoter

13X repeated p21-p53 response element

Available Since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR

Plasmid

#111177

Purpose

Tet-ON miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-SopF

Plasmid

#135174

Purpose

Expresses an mCherry-SopF fusion in mammalian cells

Depositor

Leigh Knodler

Article

Lau et al PLoS Pathog. 2019 Jul 24;15(7):e1007959.

Insert

SopF (STM1239 Salmonella enterica serovar Typhimurium)

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold

Plasmid

#212017

Purpose

mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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xCas9 3.7

Plasmid

#108379

Purpose

Mammalian expression vector for xCas9 3.7

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.7

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-VP64_Blast

Plasmid

#192650

Purpose

3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pJC175e

Plasmid

#79219

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PPSP gIII, luxAB

Expression

Bacterial

Promoter

PPSP

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.cytGAP

Plasmid

#78734

Purpose

Expression of Ca2+ sensor GAP in the cytosol

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

cytGAP

Tags

luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.nucGAP

Plasmid

#78736

Purpose

Expression of Ca2+ sensor GAP in the nucleus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

nucGAP

Tags

nucleoplasmin (Xenopus)

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pECFP-Rac1Q61L

Plasmid

#105292

Purpose

Constitutively active Rac 1

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Rac1 (RAC1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Q61L

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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