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pBbA14c-RFP

Plasmid

#126099

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA13k-RFP

Plasmid

#126092

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA12k-RFP

Plasmid

#126091

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23151

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA11k-RFP

Plasmid

#126090

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23150

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA10k-RFP

Plasmid

#126089

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23116

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA9k-RFP

Plasmid

#126088

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23113

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH7-8

Plasmid

#124991

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH7-8 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-8 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA10a-RFP

Plasmid

#126083

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23116

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH7-11

Plasmid

#124993

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH7-11 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-11 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH7-9

Plasmid

#124992

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH7-9 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-9 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH7-6

Plasmid

#124990

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH7-6 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-6 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-3

Plasmid

#124983

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-3 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

TCR-HH5-3

Use

retroviral

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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LIII 1-6 Amp RC

Plasmid

#104530

Purpose

LIII backbone vector

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Type

Empty backbone

Use

Ralstonia solanacearum transformation vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663K

Plasmid

#92239

Purpose

Express R663K mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663K mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Lysine

Promoter

T7 promoter

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-W695del

Plasmid

#92241

Purpose

Express W695-deletion mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-W695deletion mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Tryptophan 695 deleted

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663Q

Plasmid

#92240

Purpose

Express R663Q mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663Q mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Glutamine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-H698A

Plasmid

#92237

Purpose

Express H698A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-H698A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Histidine 698 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663A

Plasmid

#92238

Purpose

Express R663A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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SpDJ-1 pET15b

Plasmid

#60689

Purpose

Recombinant protein expression of DJ-1

Depositor

Mark Wilson

Article

Madzelan et al FEBS J. 2012 Nov;279(22):4111-20. doi: 1

Insert

DJ-1 (SPAC22E12.03c Fission Yeast)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pWZ414-F48

Plasmid

#23072

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Glutamine, H3 K14…

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only