We narrowed to 32,169 results for: uros

1. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

   Plasmid

   #165086

   Purpose

   gRNA 2 of pair C for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-3xFLAG-Puro PAK5 S573N

   Plasmid

   #167195

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 S573N

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 S573N
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIVTR(T7G)-PuroR-F2A-mClover3

   Plasmid

   #172291

   Purpose

   In vitro transcription of PuroR-F2A-mClover3 synthetic construct

   Depositor

   Patrik Verstreken

   Insert

   PuroR-F2A-mClover3
   

   Use

   Other

   Promoter

   T7 promoter G-initiating

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE-2xFLAG-2xSTREP-CCND1-Puro

   Plasmid

   #172634

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLD-puro-Cn-VA-NHEJ1-S262E/S263E

   Plasmid

   #141342

   Purpose

   Lentiviral vector for expression of human NHEJ1-S262E/S263E in mammalian cells

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   NHEJ1-S262E/S263E (NHEJ1 Human)
   

   Use

   Lentiviral

   Tags

   VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

   Expression

   Mammalian

   Mutation

   S262E/S263E

   Promoter

   CMV

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLD-puro-Cn-VA-NHEJ1-S262A/S263A

   Plasmid

   #141343

   Purpose

   Lentiviral vector for expression of human NHEJ1-S262A/S263A in mammalian cells

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   NHEJ1-S262A/S263A (NHEJ1 Human)
   

   Use

   Lentiviral

   Tags

   VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

   Expression

   Mammalian

   Mutation

   S262A/S263A

   Promoter

   CMV

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-PGK-RAP2A-V12-puro

   Plasmid

   #156171

   Purpose

   Lentiviral vector for expression of RAP2A-V12, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Valine

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-PGK-RAP2A-N17-puro

   Plasmid

   #156172

   Purpose

   Lentiviral vector for expression of RAP2A-N17, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   changed Serine 17 to Asparagine

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPaint-mScarlet-F-mgp100-PuroR [M1G]

   Plasmid

   #171811

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-mgp100-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPaint-mScarlet-F-hgp100-PuroR [M1G]

    Plasmid

    #171810

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-hgp100-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPaint-mNeon-F-Ova-PuroR [M1G]

    Plasmid

    #171804

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-Ova-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. XLone-Puro Cas13d-eGFP U6 RUNX1 g1

    Plasmid

    #155185

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA1

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d RUNX1 gRNA1
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. XLone-Puro Cas13d-eGFP U6 RUNX1 g2

    Plasmid

    #155186

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA2

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d RUNX1 gRNA2
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. XLone-Puro Cas13d-eGFP U6 SOX17 g1

    Plasmid

    #155187

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA1

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d SOX17 gRNA1
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-3xFLAG-Puro PAK5 T538N

    Plasmid

    #167181

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 T538N

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 T538N
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-3xFLAG-Puro PAK5 V593I

    Plasmid

    #167179

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 V593I

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 V593I
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti--Puro-3xFLAG PAK5 S364L

    Plasmid

    #167168

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 S364L

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 S364L
    

    Use

    Lentiviral

    Tags

    3x flag

    Expression

    Mammalian

    Promoter

    UBC promoter

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. XLone-BSD NFIL3 T2A Puro

    Plasmid

    #140028

    Purpose

    Tunable and temporal expression control of NFIL3 and puro resistant gene

    Depositor

    Xiaoping Bao

    Insert

    NFIL3 (NFIL3 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    puromycin

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCT-CMV-copGFP-Polß(WT)-puro

    Plasmid

    #128665

    Purpose

    mammalian expression of copGFP-Polß(WT) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    copGFP

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-EF1a-KIF5AR280H-HA-IRES-Puro

    Plasmid

    #166953

    Purpose

    Lentiviral plasmid expressing HA-tagged KIF5A R280H protein with IRES-Puro from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A-R280H (Kif5a Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    KIF5A-R280H

    Promoter

    eF1a

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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