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CROP_C9_Puro_CSF1R

Plasmid

#183277

Purpose

All-in-One CRISPRko system with a guide RNA that targets CSF1R gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CSF1R

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK2 Y261A

Plasmid

#8977

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

Y261A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_YFP

Plasmid

#45295

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

YFP

Use

Lentiviral

Expression

Mammalian

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_JAK2

Plasmid

#183303

Purpose

All-in-One CRISPRko system with a guide RNA that targets JAK2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

JAK2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK2 L232A

Plasmid

#8976

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

L232A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pGEX-2T SHP1 CS

Plasmid

#8595

Depositor

Ben Neel

Article

Timms et al Mol Cell Biol 1998 Jul;18(7):3838-50.

Insert

SHP1 (PTPN6 Human)

Tags

GST

Expression

Bacterial

Mutation

C453S (catalytically inactive)

Available Since

June 21, 2005

Availability

Academic Institutions and Nonprofits only
JAB2098

Plasmid

#190340

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt

Expression

Plant

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-mRuby3-FTL

Plasmid

#192805

Purpose

Stable expression of mRuby3-FTL in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

FTL (FTL Human)

Tags

mRuby3

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 S204A

Plasmid

#27114

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

S204A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pMS33: pTwinStrep-SUMO-PmcTnsD

Plasmid

#168166

Purpose

PmcTnsD expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

PmcCAST TnsD

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoAmax-BlastR

Plasmid

#152996

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

evoA-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSecEffector

Plasmid

#172891

Purpose

Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Inserts

Bxb1
TP901
TEV Display cassette
ProD promoter between anti aligned Bxb1 sites

Expression

Bacterial

Promoter

ProD, nTETO, and pBAD /AraC

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_VEGFB

Plasmid

#183328

Purpose

All-in-One CRISPRko system with a guide RNA that targets VEGFB gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

VEGFB

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_VEGFA

Plasmid

#183327

Purpose

All-in-One CRISPRko system with a guide RNA that targets VEGFA gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

VEGFA

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH_ss-SBP-EGFP

Plasmid

#172358

Purpose

Reporter to monitoring a streptavidin-binding peptide trafficking

Depositor

Guido Kroemer

Article

Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

Insert

ER importing signal sequence

Use

Lentiviral

Tags

SBP-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pFETCh_NFIL3

Plasmid

#86287

Purpose

Donor vector for 3' FLAG tag of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NFIL3 homology arms (NFIL3 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIL3_2

Plasmid

#86349

Purpose

Encodes gRNA for 3' target of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against NFIL3 (NFIL3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIL3_1

Plasmid

#86348

Purpose

Encodes gRNA for 3' target of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against NFIL3 (NFIL3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 T179V

Plasmid

#26997

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T179V

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

Plasmid

#188704

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNAs

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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