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pTrc-GWS1B

Plasmid

#239582

Purpose

High copy vector for expression of GWS1B Rubisco

Depositor

Matthew D. Shoulders

Article

McDonald et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

GWS1B Rubisco (6X his)

Tags

6X histag

Expression

Bacterial

Mutation

Wild-type

Promoter

Trc

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV.UBI.GFP.miR292T

Plasmid

#115972

Purpose

miR-292 regulated GFP expression

Depositor

Johan Jakobsson

Article

Sachdeva et al Proc Natl Acad Sci U S A. 2010 Jun 22;10

Insert

GFP.miR292T

Use

Lentiviral

Tags

GFP

Promoter

ubiquitin

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLV.UBI.GFP.miR292T

Plasmid

#115972

Purpose

miR-292 regulated GFP expression

Depositor

Johan Jakobsson

Article

Sachdeva et al Proc Natl Acad Sci U S A. 2010 Jun 22;10

Insert

GFP.miR292T

Use

Lentiviral

Tags

GFP

Promoter

ubiquitin

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pPRIME-TET-GFP-recipient

Plasmid

#11655

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pPRIME-TET-GFP-recipient

Plasmid

#11655

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam E2F6

Plasmid

#37965

Depositor

Jacqueline Lees

Article

Trimarchi et al Proc Natl Acad Sci U S A. 2001 Feb 13;98

Insert

E2F6 (E2F6 Human)

Expression

Mammalian

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam E2F6

Plasmid

#37965

Depositor

Jacqueline Lees

Article

Trimarchi et al Proc Natl Acad Sci U S A. 2001 Feb 13;98

Insert

E2F6 (E2F6 Human)

Expression

Mammalian

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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pEK19

Plasmid

#210578

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

BdBIP1pro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEK19

Plasmid

#210578

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

BdBIP1pro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-INPP5E

Plasmid

#171948

Purpose

Expresses INPP5E in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

INPP5E (INPP5E Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-INPP5E

Plasmid

#171948

Purpose

Expresses INPP5E in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

INPP5E (INPP5E Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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Luc-SIRT1 3'UTR

Plasmid

#20379

Depositor

Charles Lowenstein

Article

Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

Insert

SIRT1 (SIRT1 Human)

Use

Luciferase

Mutation

3'UTR of SIRT1

Available Since

May 1, 2009

Availability

Academic Institutions and Nonprofits only
Luc-SIRT1 3'UTR

Plasmid

#20379

Depositor

Charles Lowenstein

Article

Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

Insert

SIRT1 (SIRT1 Human)

Use

Luciferase

Mutation

3'UTR of SIRT1

Available Since

May 1, 2009

Availability

Academic Institutions and Nonprofits only
pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA

Plasmid

#52549

Purpose

transgenic construct for expression of GFP and Flpo

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP
Flpo recombinase

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA

Plasmid

#52549

Purpose

transgenic construct for expression of GFP and Flpo

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP
Flpo recombinase

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-EGFP-6H

Plasmid

#187001

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-EGFP-6H

Plasmid

#187001

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_Puro_sgAAVS1

Plasmid

#187818

Purpose

pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_Puro_sgAAVS1

Plasmid

#187818

Purpose

pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24ΔRING

Plasmid

#28140

Depositor

Michelle Barton

Article

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Mutation

Deletion of the RING domain: aa 1-139

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only