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T7-PseB-His6

Plasmid

#89723

Purpose

Expresses PseB from C. jejuni with an N-terminal T7 tag and a C-terminal His6 tag

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseB

Tags

His6

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDSAY

Plasmid

#62291

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-shcIAP1A

Plasmid

#44129

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

cIAP1

Use

Lentiviral and RNAi

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
pSM038

Plasmid

#128293

Purpose

asymmetric plasmid partitioning E. coli - helper plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

PphlF - sfyfp-P2A-motA

Expression

Bacterial

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSPB866

Plasmid

#128304

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - GCN4zip.MSE-cfp-parB

Expression

Bacterial

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_P3-ehaA_LacI

Plasmid

#186315

Purpose

Plasmid for inducible E coli expression of the P3 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

P3-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_AP4-ehaA_LacI

Plasmid

#186316

Purpose

Plasmid for inducible E coli expression of the AP4 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

AP4-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRGD-HmR

Plasmid

#74107

Purpose

Plasmid containing hygromycin B resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

hph5

Use

Bacterial vector based on pmb1 replicon

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pEGF/HisB-cGK1

Plasmid

#106451

Purpose

expression of murine cyclic GMP-dependent kinase

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

cyclic GMP-dependent kinase (Prkg1 Mouse)

Tags

6xHis

Expression

Mammalian

Mutation

WT

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDCAF-ABC

Plasmid

#113652

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA and ndmB and ndmC

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDL14

Plasmid

#17837

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

gpdA promoter, lacZ, trpC

Use

Synthetic Biology; A. nidulans

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pGEM cWnt14 (CT#692)

Plasmid

#13947

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

Wnt-14 (WNT9A Chicken)

Expression

Bacterial

Mutation

Note that this insert is in the reverse direction…

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pmRuby2-IRES-Clover

Plasmid

#171059

Purpose

Expresses mRuby2 and mClover2 in the cytoplasm as negative control for FRET.

Depositor

Ke Xu

Article

Chen et al Light Sci Appl. 2021 May 8;10(1):97. doi

Insert

IRES

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSARSF534-3

Plasmid

#45618

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaEC35-GFP_C

Tags

H6

Expression

Bacterial

Mutation

SYG->CYG in chromophore

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pDL11

Plasmid

#17832

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

alcA promoter, lacZ, argB,

Use

Synthetic Biology; Aspergillus nidulans expression

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pDSAG

Plasmid

#62289

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, GFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCC48

Plasmid

#128285

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, BBa_J23100 - phlF, parS

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDSARN

Plasmid

#62295

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDSAT

Plasmid

#62290

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, mTurquoise CFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDL13

Plasmid

#17836

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

alcA promoter, lacZ, trpC

Use

Synthetic Biology; A. nidulans

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only