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lifeact-mNeongreen-pA

Plasmid

#241202

Purpose

Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

lifeact-mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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T2A-mCherry-P2A-NTRv2-pA-ORF-1

Plasmid

#241196

Purpose

Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry; NTR-v2

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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T2A-mCherry-P2A-NTRv2-pA-ORF-2

Plasmid

#241197

Purpose

Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry; NTR-v2

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-lifeact-mNeongreen-ORF-3

Plasmid

#241174

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-mNeongreen-ORF1

Plasmid

#241176

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
linker-P2A-mNeongreen-ORF2

Plasmid

#241177

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
linker-P2A-mNeongreen-ORF3

Plasmid

#241178

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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FRT-acrymCherry-FRT

Plasmid

#241179

Purpose

Middle entry vector for mini-Golden subcloning platform; acry-mCherry

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

acry-mCherry

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1369

Plasmid

#239828

Purpose

WT OrufIscB mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1623

Plasmid

#239833

Purpose

WT OrufIscB E. coli expression for recombinant purification

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1376

Plasmid

#239829

Purpose

WT OrufIscB omegaRNA mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB omegaRNA scaffold

Expression

Mammalian

Promoter

human U6

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_BFP-P2A-NTRv2-pA

Plasmid

#200538

Purpose

mini circle middle entry plasmid for BFP-P2A-NTR version 2-pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-EGFP-pA ORF-3 without ATG

Plasmid

#200519

Purpose

mini circle middle entry plasmid for 2a-eGFP-pA ORF3 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

eGFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_memb-EGFP-pA

Plasmid

#200520

Purpose

Mini circle middle entry plasmid for membrane GFP- pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

memb-GFP

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-mCherry-pA ORF-1 with ATG

Plasmid

#200522

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 1 (with ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-mCherry-pA ORF-2 with ATG

Plasmid

#200523

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 2 (with ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-mCherry-pA ORF-3 with ATG

Plasmid

#200524

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 3 (with ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-ME_2a-mCherry-pA ORF-1 without ATG

Plasmid

#200525

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 1 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-ME_2a-mCherry-pA ORF-2 without ATG

Plasmid

#200526

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 2 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-ME_2a-mCherry-pA ORF-3 without ATG

Plasmid

#200527

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 3 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge