Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 4,802 results for: pcr

Clear keywords
Showing: 2921 - 2940 of 4802 results

  1. KISSPEPTIN-R-DuET

    Plasmid
    #213328
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    KISSPEPTIN-R (KISS1R Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213328-plasmid-map-sequence-id-430862

    Enlarge

  2. HTR1D-DuET

    Plasmid
    #213318
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR1D (HTR1D Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213318-plasmid-map-sequence-id-430855

    Enlarge

  3. pAAV-hSyn-GRAB_CRFmut

    Plasmid
    #208659
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in neurons
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    Use
    AAV
    Promoter
    hSyn
    Available Since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    208659-plasmid-map-sequence-id-410350

    Enlarge

  6. MAS1L-Tango

    Plasmid
    #66426
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MAS1L (MAS1L Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. Lmx1a-in situ probe

    Plasmid
    #45071
    Depositor
    Kathleen Millen
    Insert
    Lmx1a LIM homeobox transcription factor 1 alpha (residues 94-1149) (Lmx1a Mouse)
    Expression
    Mammalian
    Mutation
    Lmx1a residues 94-1149
    Available Since
    May 30, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. (P79) OCN/Wnt 10b transgene

    Plasmid
    #25878
    Depositor
    Ormond MacDougald
    Insert
    Wnt10b (Wnt10b Mouse)
    Use
    Mouse Targeting
    Tags
    B-globin intron/poly A coding sequence and OCN pr…
    Expression
    Mammalian
    Available Since
    Oct. 21, 2010
    Availability
    Academic Institutions and Nonprofits only

  9. pAAV-anti-VEGF(Nb35) PAGER(TF)

    Plasmid
    #230000
    Purpose
    Expresses anti-VEGF PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-Nb35-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230000-plasmid-map-sequence-id-457212

    Enlarge

  10. pAAV-anti-mCherry(LaM8) PAGER(TF)

    Plasmid
    #229999
    Purpose
    Expresses anti-mCherry PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-LaM8-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229999-plasmid-map-sequence-id-457197

    Enlarge

  11. GABBR1-DuET

    Plasmid
    #213247
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GABBR1 (GABBR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213247-plasmid-map-sequence-id-430899

    Enlarge

  12. AVPR2-DuET

    Plasmid
    #213188
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    AVPR2 (AVPR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213188-plasmid-map-sequence-id-430753

    Enlarge

  14. OXGR1 -Tango

    Plasmid
    #66466
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    OXGR1 (OXGR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. MCHR2-DuET

    Plasmid
    #213338
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MCHR2 (MCHR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213338-plasmid-map-sequence-id-430867

    Enlarge

  16. GPR120-DuET

    Plasmid
    #213261
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR120 (FFAR4 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213261-plasmid-map-sequence-id-430811

    Enlarge

  17. CCR4-DuET

    Plasmid
    #213200
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR4 (CCR4 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213200-plasmid-map-sequence-id-430763

    Enlarge

Showing: 2921 - 2940 of 4802 results