163,268 results

1. pTubb3-MC

   Plasmid

   #87112

   Purpose

   minicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. nesprin TSmod 25

   Plasmid

   #125555

   Purpose

   Nesprin-2G force sensor with cerulean-F25-venus TSmodule

   Depositor

   Daniel Conway

   Article

   Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

   Insert

   mini-nesprin-2G (Syne2 Mouse)
   

   Use

   Tags

   cerulean, venus

   Expression

   Mammalian

   Mutation

   TSmod changed from mTFP to cerulean, flageliform …

   Promoter

   CMV

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. SP-dCas9-VPR

   Plasmid

   #63798

   Purpose

   SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   SP-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL3.7-EF-EYFP-YAP1_5SA-PolyA

   Plasmid

   #112285

   Purpose

   Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Human YAP1-2gamma with 5 Serines (61, 109, 127, 1…

   Promoter

   EF-1 alpha

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pROSA26-ERT2CreERT2

   Plasmid

   #149436

   Purpose

   ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

   Depositor

   Primo Schaer

   Article

   Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

   Insert

   ERT2-Cre-ERT2 fusion protein
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   ERT2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDB_347

   Plasmid

   #231114

   Purpose

   CBE activity-based selection cloning vector

   Depositor

   David Root, John Doench

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-HA-GFP

   Plasmid

   #22612

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   GFP
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Dec. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. LZRS-DNMT1

   Plasmid

   #24952

   Depositor

   Paul Khavari

   Article

   Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

   Insert

   DNMT1 (DNMT1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHis-hTG2

   Plasmid

   #100719

   Purpose

   Expression of human transglutaminase 2 in E.coli

   Depositor

   Byung Il Lee

   Article

   Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

   Insert

   TGM2 (TGM2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   silent mutation Arg580 (AGG--CGT) to enhance expr…

   Promoter

   T7

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-FLEX-EGFPL10a (AAV Retrograde)

    Viral Prep

    #98747-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Eric Schmidt, Nathaniel Heintz, Alexander Nectow

    Article

    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

    Promoter

    EF1a

    Tags

    EGFP (Cre-dependent)

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only