We narrowed to 19,699 results for: ACE

1. pZW6 (CK2alpha)

   Plasmid

   #27086

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha (CSNK2A1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDB1 (CK2alpha)

   Plasmid

   #27083

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha (CSNK2A1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX-IRES-Hygro_GCaMP6s

   Plasmid

   #164591

   Purpose

   Stable integration and expression of GCaMP6s in mammalian cells

   Depositor

   Joseph Hyser

   Article

   Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

   Insert

   GCaMP6s
   

   Use

   Lentiviral

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTarget

   Plasmid

   #186452

   Purpose

   used as source for acquisition of new spacers

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAB46 (CK2beta)

   Plasmid

   #27085

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2beta (CSNK2B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX330-CENPA

   Plasmid

   #227282

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of CENPA (CENPA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSF-G1(7FTrp)RS

   Plasmid

   #177310

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZW16 (CK2alpha')

   Plasmid

   #27087

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha' (CSNK2A2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Emerin pEGFP-N2 (588)

   Plasmid

   #61985

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Emerin (EMD Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBAD-iLACCO1

    Plasmid

    #218817

    Purpose

    Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAD-iLACCO1.1

    Plasmid

    #218818

    Purpose

    Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with low affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBAD-iLACCO1.2

    Plasmid

    #218819

    Purpose

    Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1.2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBAD-DiLACCO1

    Plasmid

    #218820

    Purpose

    Bacterial expression of genetically encoded green fluorescent L-lactate control biosensor

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    DiLACCO1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GW1-rogRFP2

    Plasmid

    #207876

    Purpose

    Mammalian expression of the red fluorescent rogRFP2 redox sensor

    Depositor

    Mathew Tantama

    Article

    Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

    Insert

    rogRFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p11d-aRPA (MSW#712)

    Plasmid

    #208078

    Purpose

    Expression of Alternative human Replication Protein A (RPA1,4,3) in E. coli

    Depositor

    Marc Wold

    Article

    Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

    Insert

    RPA1, RPA4, RPA3 (RPA1, RPA3, RPA4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Rabbit GFP Cav 2.2 pMT2

    Plasmid

    #58737

    Purpose

    expression of GFP tagged rabbit Cav2.2 in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

    Insert

    Cav2.2 (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-ExRai-AMPKAR(T/A)

    Plasmid

    #192447

    Purpose

    Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR(T/A)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Thr 6 in AMPK substrate mutated to Ala.

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. WT C-His Ana GvpC in pET28a

    Plasmid

    #85732

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEM-Cas9HF1

    Plasmid

    #89961

    Purpose

    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    Cas9HF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP-N-Ezrin

    Plasmid

    #202684

    Purpose

    fluorescent protein tagged ezrin

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    Mus musculus EZR(1-586):EGFP (Ezr Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA-iLACCO1

    Plasmid

    #218821

    Purpose

    Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCRISPR-mcBEST

    Plasmid

    #209416

    Purpose

    Plasmid for multiplexed cytosine base editing in streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

    Inserts

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    csy4 from Pseudomonas aeruginosa PAO1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    base editing cassette: PtipA ; sgRNAs: PkasO* an…

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CMV PGK1-3xHA

    Plasmid

    #220913

    Purpose

    Expresses PGK1-3xHA

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1-3xHA (PGK1 Human)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTU1-B-SP44s-GUS

    Plasmid

    #172430

    Purpose

    Expresses Streptomyces codon-optimised beta-D-glucuronidase

    Depositor

    Simon Moore, Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Insert

    beta-D-glucuronidase
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    SP44s

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GCaMP-AMPK-SPARK

    Plasmid

    #226257

    Purpose

    Dual reporter of Ca2+ levels and AMPK activity

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    GCaMP-AMPK-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pDEST exorh:mGreenLantern

    Plasmid

    #195988

    Purpose

    Transgenesis backbone

    Depositor

    Nicolas Rohner, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CMV-synaptophysin-Lime

    Plasmid

    #201016

    Purpose

    Expresses Lime in synaptic vesicle lumen by fusion to synaptophysin

    Depositor

    Jaime de Juan-Sanz

    Article

    Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

    Insert

    Synaptophysin-Lime (Syp Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD-Padron2

    Plasmid

    #172355

    Purpose

    Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

    Depositor

    Stefan Jakobs

    Article

    Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

    Insert

    Padron2
    

    Use

    Tags

    6xHisTag (N terminal on backbone)

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BE4

    Plasmid

    #100802

    Purpose

    Expresses BE4 in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

    Insert

    BE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FUGW-Golgi-TurboID

    Plasmid

    #200964

    Purpose

    3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Golgi-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCas9(D10A)

    Plasmid

    #74495

    Purpose

    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

    Insert

    Cas9 D10A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    pTeto

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA-DiLACCO1

    Plasmid

    #218824

    Purpose

    Mammalian expression of genetically encoded green fluorescent L-lactate control biosensor

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    DiLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBTK301

    Plasmid

    #110593

    Purpose

    BTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Bba_B0015 Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. CanlonicSF

    Plasmid

    #178342

    Purpose

    To explore lactate dynamics in intact systems

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    CanlonicSF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEGFP-hsRPA32 (MSW#497)

    Plasmid

    #208068

    Purpose

    Expression of GFP-tagged hsRPA2 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

    Insert

    RPA2 (RPA2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T88I- please see depositor comments

    Promoter

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET21 gamma-tubulin

    Plasmid

    #101825

    Purpose

    production of gamma-tubulin in bacteria

    Depositor

    Maria Alvarado-Kristensson, Ana Carrera

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Whole protein

    Promoter

    T7

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET26b_Nb.AD101

    Plasmid

    #131402

    Purpose

    E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody AD101
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCRISPathBrick

    Plasmid

    #65006

    Purpose

    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive native promoters

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127321

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR

    Plasmid

    #176311

    Purpose

    Expresses NLS-pMag-CreC60 with tagBFP marker

    Depositor

    Yingxiao Wang

    Article

    Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

    Insert

    tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1alpha

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge