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miR21-IRE-TREAT

Plasmid

#196930

Purpose

mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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Let7-IRE-TREAT

Plasmid

#196925

Purpose

Let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::NeoR-bGHpA

Plasmid

#62438

Purpose

MXS_chaining vector with PGK::NeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Neomycin with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pT2P-EF-Q7mu

Plasmid

#129303

Purpose

Positive control for ATP measurement, mitochondria outer membrane, Tol2 transposon

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-7mu

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205542

Purpose

Express EPN1-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast

Plasmid

#205547

Purpose

Express EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11-MCP-I

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast

Plasmid

#205548

Purpose

Express EPN11-MCP-p6-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11-MCP-p6

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pOIMars1CyN

Plasmid

#75221

Purpose

Mammalian cell surface expression of fluorogen activating protein Mars1Cy

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

ss-HA-Mars1Cy-CD80(237-306)

Tags

HA epitope

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pFH2.96_PB-EPN1-T2A-eGFP-PGK-Blast

Plasmid

#205541

Purpose

Express EPN1-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.100_PB-EPN11-T2A-eGFP-PGK-Blast

Plasmid

#205545

Purpose

Express EPN11-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pSIF-H1-hpolb-copGFP

Plasmid

#23256

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

Insert

DNA polymerase beta shRNA

Use

Lentiviral and RNAi

Tags

GFP

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
AY10_pS.Donor.R5.TS

Plasmid

#100292

Purpose

CCR5-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

18 bp RFLP (MluI+KspAI)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-2x

Plasmid

#172282

Purpose

Expressing EGFP mRNA fused with 2 tandem repeats of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-2x

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-4x

Plasmid

#172283

Purpose

Expressing EGFP mRNA fused with 4 tandem repeats of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-4x

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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sm-9xGCN4-Renilla-MS2

Plasmid

#178009

Purpose

Expresses Spaghetti Monster (sm) 9xGCN4-Renilla mRNA reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Gomez-Puerta et al Elife. 2022 Jun 22;11. pii: 75580. doi:

Insert

sm-9xGCN4

Expression

Bacterial and Mammalian

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-pEF1a-rtTAc

Plasmid

#172121

Purpose

Constitutive expression of InteinC-rtTAC

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

InteinC-rtTAc

Use

AAV

Promoter

EF1a

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 S208A

Plasmid

#27115

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

S208A

Available Since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
Nano-lantern(Ca2+)_600/pRSETB

Plasmid

#51976

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-2G

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
sspa-GCaMP6s

Plasmid

#67559

Purpose

short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6s

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
IRE-TPI-wildtype-TREAT

Plasmid

#196923

Purpose

The coding sequence of Triose phosphate isomerase gene was fused downstream Renilla in the ORF

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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