We narrowed to 8,887 results for: PAN

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_YES_i2

Plasmid

#231419

Purpose

This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP
sgRNA-Y

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_i1

Plasmid

#231416

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pRDB_268

Plasmid

#231112

Purpose

SpG-Cas9

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

Cas9-SpG

Use

CRISPR and Lentiviral

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA516

Plasmid

#231118

Purpose

Fragmid fragment: (2A Selection) CBE activity-based selection intron

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

PuroN'-intron-PuroC'

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA566

Plasmid

#231115

Purpose

Fragmid fragment: (guide cassette) ABE activity-based selection cloning

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA568

Plasmid

#231117

Purpose

Fragmid fragment: (guide cassette) CBE activity-based selection cloning

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA509

Plasmid

#231116

Purpose

Fragmid fragment: (2A Selection) ABE activity-based selection intron

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

PuroN'-intron-PuroC'

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_eGFP-pScaC-MTS

Plasmid

#228827

Purpose

For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC C-terminally fused to a mitochondrial targeting sequence in HeLa Flp-In cells

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Tags

MTS and eGFP

Expression

Mammalian

Mutation

aa 33-223

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2

Plasmid

#228829

Purpose

Insect cell expression of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET-MhCINS-6xHis

Plasmid

#194304

Purpose

Bacterial expression of MhCINS protein with a C terminal His-tag and transit peptide removed at N terminal

Depositor

Richard Saunders

Article

Liu et al J Ecology 2024; 00, 1–20. doi: 10.1111/1

Insert

MhCINS

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAM248

Plasmid

#226892

Purpose

6xHis-PSP-GS-PANN(75-150)-(5aaGS)-Msp1(36-362)_W166A/Y167A

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Tags

His-Precission-PANN

Expression

Bacterial

Mutation

W166A/Y167A

Promoter

T7

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-D134*

Plasmid

#191110

Purpose

An E. coli sfGFP reporter with one TAG at position 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Mutation

changing D134 to TAG

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-V2*D134*

Plasmid

#191111

Purpose

An E. coli sfGFP reporter with two TAG at positions 2 & 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Mutation

changing V2 & D134 to TAG

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAM245

Plasmid

#226889

Purpose

6xHis-PSP-GS-PANN(75-150)-(5aaGS)-Msp1(36-362)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Tags

His-Precission-PANN

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pEC2796 (pSpy0C4)

Plasmid

#220280

Purpose

integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombination

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMK454 (dTAG-BSD)

Plasmid

#214384

Purpose

dTAG for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

dTAG-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSM045

Plasmid

#217399

Purpose

KmR, pBTRcK broad host range vector with BTE1(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

Depositor

Brian Pfleger

Article

Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

Insert

BTE1

Expression

Bacterial

Promoter

PTrc

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSM046

Plasmid

#217400

Purpose

KmR, pBTRcK broad host range vector with BTE2(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

Depositor

Brian Pfleger

Article

Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

Insert

BTE2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTrc

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMS161

Plasmid

#215683

Purpose

Guide only plasmid targeting chrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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