We narrowed to 40,715 results for: Spr

1. pXPR_501

   Plasmid

   #96922

   Purpose

   for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64

   Depositor

   John Doench, David Root

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0045-EF1a-PguCas13b-NES-HIV

   Plasmid

   #103861

   Purpose

   Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PguCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. p415-GalL-Cas9-CYC1t

   Plasmid

   #43804

   Purpose

   A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   Human Optimized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GalL

   Available since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-SMVP-Cas9N-U6-gRNA M3

   Plasmid

   #80936

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC0052-REPAIR non-targeting guide clone into pC0043

   Plasmid

   #103868

   Purpose

   Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   non-targeting guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ153

   Plasmid

   #69304

   Purpose

   Gateway entry vector with pco-dCas9-3X(SRDX)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pco-dCas9-3X(SRDX) (Plant codon-optimized)
   

   Use

   CRISPR

   Tags

   2XFLAG

   Expression

   Mutation

   SRDX (X3) repressor fusion; NLS fusion

   Promoter

   Available since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCE048-SiT-Cas12a

   Plasmid

   #128124

   Purpose

   Express both AsCas12a and CRISPR arrays on single transcripts

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-Triplex
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAS9counter

   Plasmid

   #107192

   Purpose

   Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   Cas9
   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   prab17

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-BE-zCas9

   Plasmid

   #101739

   Purpose

   expression of an optimized Cas9 for base-editing in zebrafish

   Depositor

   Shuo Lin

   Article

   Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

   Insert

   rAPOBEC1-XTEN-zCas9-UGI-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CMV

   Available since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p201H Cas9

    Plasmid

    #59176

    Purpose

    Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    hph
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    2x35S and StUbi-3P

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC0044-EF1a-RanCas13b-NES-mapk

    Plasmid

    #103855

    Purpose

    Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    RanCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and mapk NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPBO.ACT001

    Plasmid

    #182711

    Purpose

    aTc inducible dCasRx

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    2x-FLAG

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-idCas9-vpr

    Plasmid

    #89985

    Purpose

    inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

    Depositor

    Jie Na

    Article

    Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

    Insert

    dCas9-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHsp70-Cas9

    Plasmid

    #45945

    Purpose

    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

    Depositor

    Jill Wildonger, Kate O'Connor-Giles, Melissa Harrison

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x FLAG

    Expression

    Mutation

    Silent change from C to A at bp 834 of insert

    Promoter

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV hUbC-dCas9-T2A-GFP

    Plasmid

    #53191

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEn-Chimera

    Plasmid

    #61432

    Purpose

    Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGuide

    Plasmid

    #64711

    Purpose

    Cloning and expression of Streptococcus pyogenes guide RNA

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Streptococcus pyogenes guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3s-Sniper-Cas9

    Plasmid

    #113912

    Purpose

    Expresses Sniper-Cas9 with CMV promoter

    Depositor

    Jungjoon Lee

    Article

    Lee et al Nat Commun. 2018 Aug 6;9(1):3048. doi: 1

    Insert

    Sniper-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC18-mini-Tn7T-Lac-dCas9 (Ptac)

    Plasmid

    #105234

    Purpose

    Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens

    Depositor

    Kristala Prather

    Article

    Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

    Insert

    S. pasteurianus dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Bacterial

    Mutation

    D10A, H599A

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-LMNA-gRNA1

    Plasmid

    #122507

    Purpose

    expresses WT spCas9 and a chimeric gRNA targeting human LMNA

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    LMNA-gRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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