1. p426 46Q GPD

   Plasmid

   #1182

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 46Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pEGFP-n1-APP

   Plasmid

   #69924

   Purpose

   mammalian expression of human APP fused to EGFP

   Depositor

   Zita Balklava, Thomas Wassmer

   Article

   Currinn et al Cell Mol Life Sci. 2015 Jul 28.

   Insert

   APP (APP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDEST_FLAG-HUWE1_C4341S

   Plasmid

   #187156

   Purpose

   mammalian cell expression FLAG-HUWE C4341S

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   C4341S

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDARMO_CMVT_FLAG-HUWE1

   Plasmid

   #187121

   Purpose

   mammalian cell expression of FLAG-HUWE1

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. hWIP1 FLAG

   Plasmid

   #28105

   Depositor

   Lawrence Donehower

   Article

   Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

   Insert

   WIP1 (PPM1D Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HUWE1-sgRNA-1

   Plasmid

   #86924

   Purpose

   To express sgRNA target HUWE1 gene

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   HUWE1 sgRNA1 (HUWE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

6. HUWE1-sgRNA-2

   Plasmid

   #86925

   Purpose

   To express sgRNA target HUWE1 gene

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   HUWE1 sgRNA2 (HUWE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

7. Human HUWE1 (3993-4374)

   Plasmid

   #110754

   Purpose

   Bacterial Expression of HUWE1 HECT domain

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Dead Streptavidin-SpyCatcher

   Plasmid

   #59547

   Purpose

   pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Dead-dloop-Streptavidin-SpyCatcher
   

   Use

   Tags

   N-terminally shortened SpyCatcher

   Expression

   Bacterial

   Mutation

   PolyAsp in L3/4 loop for ion-exchange

   Promoter

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pEYFP-C1-hSERT

   Plasmid

   #70103

   Purpose

   mammalian expression of hSERT with N-terminal YFP fusion

   Depositor

   Harald Sitte

   Article

   Sucic et al J Biol Chem. 2010 Apr 2;285(14):10924-38

   Insert

   SERT (SLC6A4 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pECFP-C1-hSERT

    Plasmid

    #70104

    Purpose

    mammalian expression of hSERT with N-terminal CFP fusion

    Depositor

    Harald Sitte

    Article

    Sucic et al J Biol Chem. 2010 Apr 2;285(14):10924-38

    Insert

    SERT (SLC6A4 Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-P1-LoxP-Neo-N-miRFP670-C

    Plasmid

    #159434

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Inserts

    Neomycin
    miRFP670
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Expression

    Mutation

    Promoter

    SV40

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAVA-LGF2-Gal11p

    Plasmid

    #127481

    Purpose

    Positive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    LGF2-Gal11p (GAL11 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAVA-Gal11p-LGF2

    Plasmid

    #127480

    Purpose

    Positive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    Gal11p-LGF2 (GAL11 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDARMO_CMVT_3xFLAG-HUWE1_R4187C

    Plasmid

    #187142

    Purpose

    mammalian cell expression of 3xFLAG-HUWE1 R4187C

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R4187C

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5/FRT/TO HIS HSPA14

    Plasmid

    #19542

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 14 (HSPA14 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST_FLAG-HUWE1_YH/GG

    Plasmid

    #187123

    Purpose

    mammalian cell expression of FLAG-HUWE1 Y355G/H356G

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Y355G/H356G

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDARMO_CMVT_3xFLAG-HUWE1_H3962D

    Plasmid

    #187131

    Purpose

    mammalian cell expression of 3xFLAG-HUWE1 H3962D

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    H3962D

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF-TAZ-N-Flag (-4)

    Plasmid

    #40914

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Vector has no SV40 origin, and lacks the final fo…

    Promoter

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pDONR221-HUWE1_UIM-mut

    Plasmid

    #187147

    Purpose

    Entry clone

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Entry clone

    Tags

    Expression

    Mutation

    A1376G/S1382A

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST_FLAG-HUWE1_F3194S

    Plasmid

    #187140

    Purpose

    mammalian cell expression of FLAG-HUWE1 F3194S

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    F3194S

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge