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Plasmid#157950PurposeLentiviral expression of AID-BEmaxDepositorInsertLenti AID-BEmax
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha Ba-crystallin 3kb promoter/AcGFP
Plasmid#98097PurposeaBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha Ba-crystallin 3 kb fragment (-3000/-1)Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2D-A
Plasmid#221713PurposeExpression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2D-A (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationD683A in active sitePromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG3-drAgo2DY-EF
Plasmid#221733PurposeExpression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.DepositorInsertdrAgo2DY-EF (ago2 Zebrafish)
UseTags3xFLAGExpressionMammalianMutationD651E and Y680F in active sitePromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2F-Y
Plasmid#221722PurposeExpression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2F-Y (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationF666Y in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2E-D
Plasmid#221721PurposeExpression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2E-D (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationE637D in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2D-A
Plasmid#221720PurposeExpression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2D-A (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationD669A in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2
Plasmid#221719PurposeExpression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2 (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationPromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2DY-EF
Plasmid#221716PurposeExpression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2DY-EF (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationD651E and Y680F in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2Y-F
Plasmid#221715PurposeExpression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2Y-F (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationY680F in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2D-E
Plasmid#221714PurposeExpression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2D-E (ago2 Zebrafish)
UseFish expressionTags3xFLAGExpressionMutationD651E in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB153 - pL2_pSB90_2x35S::ZCT1::tMAS
Plasmid#123195Purposebinary plant vector for transient expression of ZCT1 from Catharanthus roseusDepositorInsert2x35S::ZCT1::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB160 - pL2_pSB90_2x35S::ORCA3::tMAS
Plasmid#123196Purposebinary plant vector for transient expression of ORCA3 from Catharanthus roseusDepositorInsert2x35S::ORCA3::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS
Plasmid#123199Purposebinary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)DepositorInserttMAS::rLUC-I::pMAS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB62 - pL0_STR (pro + 5U)
Plasmid#123186PurposeGolden Gate (MoClo; PRO + 5U) compatible STR1 promoter from Catharanthus roseusDepositorInsertCatharanthus roseus STR1 promoter and 5'UTR
UsePart for plant expressionTagsExpressionPlantMutationNo BpiI and BsaI sitesPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP304-pAAV-EFS-dSaCas9-VP64-pA
Plasmid#113679PurposeA EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted regionDepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Synthetic BiologyTagsNLS and VP64ExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP13A3 D498N
Plasmid#195850PurposeExpresses D498N mutant Homo sapiens ATP13A3DepositorInsertATPase 13A3 D498N (ATP13A3 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-2xTS
Plasmid#109419PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-AT1R-APEX
Plasmid#96847PurposeFor expression of AT1R-APEX in mammalian cellsDepositorInsertAT1R-APEX (AGTR1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJEP302-pAAV-CMV-dSaCas9-KRAB-pA
Plasmid#113677PurposeA CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.DepositorInsertde-catalyzed SaCas9
UseAAV and CRISPRTagsKRAB and NLSExpressionMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only