Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 409 results for: RPA;

Showing: 281 - 300 of 409 results

  1. S+12subs

    Plasmid
    #167177
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+12R
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed Q at 111 to R; A at 112 to T; E at 116to …
    Promoter
    P10 and PH
    Available Since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167177-plasmid-map-sequence-id-334485

    Enlarge

  6. A1-LCD deltahexa

    Plasmid
    #169714
    Purpose
    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, hexapeptide including aa 259-264 deleted
    Depositor
    Tanja Mittag
    Article
    Martin et al Science. 2020 Feb 7;367(6478):694-699. d
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    hnRNPA1-A Low complexity domain including amino a…
    Promoter
    T7
    Available Since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169714-plasmid-map-sequence-id-334793

    Enlarge

  7. A1-LCD+7R+12D

    Plasmid
    #178678
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+7R+12D (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Arg residues added, twelve Glu residues add…
    Promoter
    Available Since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178678-plasmid-map-sequence-id-357023

    Enlarge

  9. A1-LCD allW D262V

    Plasmid
    #234616
    Purpose
    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
    Depositor
    Tanja Mittag
    Article
    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2
    Insert
    hnRNPA1_LCD_allW D262V (HNRNPA1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    C-terminal domain (186-320) bearing familial ALS …
    Promoter
    T7
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234616-plasmid-map-sequence-id-469043

    Enlarge

  10. A1-LCD allW D262N

    Plasmid
    #234615
    Purpose
    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
    Depositor
    Tanja Mittag
    Article
    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2
    Insert
    hnRNPA1_LCD_allW D262N (HNRNPA1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    C-terminal domain (186-320) bearing familial ALS …
    Promoter
    T7
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234615-plasmid-map-sequence-id-469028

    Enlarge

  13. A1-LCD-2R-2K+3D

    Plasmid
    #178680
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with two Arg residues removed, two Lys removed, three Asp added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-2R+2K+3D (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    two Arg residues removed, two Lys added, three As…
    Promoter
    Available Since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178680-plasmid-map-sequence-id-356258

    Enlarge

  14. A1-LCD-9F+3Y

    Plasmid
    #178663
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with nine Phe residues removed, three Tyr added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-9F+3Y (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    nine Phe residues removed, three Tyr added
    Promoter
    Available Since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178663-plasmid-map-sequence-id-356435

    Enlarge

  15. A1-LCD-14N+14Q

    Plasmid
    #178692
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues replaced with Asn (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-14N+14Q (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    fourteen Gln residues replaced with Asn
    Promoter
    Available Since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178692-plasmid-map-sequence-id-356415

    Enlarge

  16. A1-LCD-10G+10S

    Plasmid
    #178682
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with ten Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-10G+10S (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    ten Gly residues replaced with Ser
    Promoter
    Available Since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178682-plasmid-map-sequence-id-356414

    Enlarge

  17. A1-LCD+7K+12D

    Plasmid
    #178679
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with seven Lys residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+7K+12D (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    seven Lys residues added, twelve Glu residues add…
    Promoter
    Available Since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178679-plasmid-map-sequence-id-356398

    Enlarge

  18. A1-LCD-30G+30S

    Plasmid
    #178684
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-30G+30S (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    thirty Gly residues replaced with Ser
    Promoter
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178684-plasmid-map-sequence-id-356310

    Enlarge

  19. A1-LCD+23G-23S

    Plasmid
    #178685
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD+23G-23S (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    twenty-three Ser residues replaced with Gly
    Promoter
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178685-plasmid-map-sequence-id-356307

    Enlarge

  20. A1-LCD-4R-2K+5D

    Plasmid
    #178681
    Purpose
    Bacterial expression of N-terminally 6His tagged A1-LCD with four Arg residues removed, two Lys removed, five Asp added (LCD: low-complexity domain, aa 186-320)
    Depositor
    Tanja Mittag
    Article
    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1
    Insert
    A1-LCD-4R+2K+5D (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    four Arg residues removed, two Lys added, five As…
    Promoter
    Available Since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178681-plasmid-map-sequence-id-356295

    Enlarge

Showing: 281 - 300 of 409 results