We narrowed to 462 results for: SK2

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pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro

Plasmid

#120562

Purpose

Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

G12D

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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R979-X58-008: Hs.KRAS4b(1-188)

Plasmid

#159691

Purpose

Baculovirus protein expression of KRAS4b (1-188)

Depositor

Dominic Esposito

Article

Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

Insert

Hs.KRAS4b(1-188) (KRAS Human)

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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PC4_3spc34h

Plasmid

#70700

Purpose

Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on spc34

Depositor

Stephen Harrison

Article

Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

Insert

DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)

Tags

his-tag on C-terminus of spc34

Expression

Bacterial

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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PC4_3dad1h

Plasmid

#70699

Purpose

Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on dad1

Depositor

Stephen Harrison

Article

Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

Insert

DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)

Tags

his-tag on C-terminus of dad1

Expression

Bacterial

Promoter

T7

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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W118-1_hRSK3(RPS6KA2)

Plasmid

#180384

Purpose

lentiviral transduction of human RSK3 (RPS6KA2) gene

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

RPS6KA2 (RPS6KA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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KRAS-V14I

Plasmid

#154427

Purpose

V14I Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

Depositor

Kenneth Westover

Article

Bera et al J Biol Chem. 2019 Sep 20;294(38):13964-1

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-CD59

Plasmid

#222307

Purpose

Synchronize the trafficking of CD59 from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and CD59 fused to SBP-EGFP (CD59 Human)

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-FRB-KRas/N12

Plasmid

#214302

Purpose

Express split-KRas fragment fused with FRB and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-KRas fragment fused with FRB (KRAS Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Promoter

Tac

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b-KRas/13C-FKBP-MBP

Plasmid

#214303

Purpose

Express split-KRas fragment fused with FKBP and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-KRas fragment fused with FKBP (KRAS Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

KRas-Q61L

Promoter

T7

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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R979-X57-008: Hs.KRAS4b(1-169)

Plasmid

#159690

Purpose

Baculovirus protein expression of KRAS4b (1-169)

Depositor

Dominic Esposito

Article

Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

Insert

Hs.KRAS4b(1-169) (KRAS Human)

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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MAP3K6 gRNA (BRDN0001162255)

Plasmid

#76012

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K6

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K6 (MAP3K6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K6 gRNA (BRDN0001144737)

Plasmid

#76013

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K6

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K6 (MAP3K6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K6 gRNA (BRDN0001146318)

Plasmid

#76014

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K6

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K6 (MAP3K6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K6 gRNA (BRDN0001162229)

Plasmid

#76015

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K6

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K6 (MAP3K6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) K-Ras(G12C)

Plasmid

#224284

Purpose

Cell transfection and expression of K-Ras (G12C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

K-Ras (KRAS Human)

Expression

Mammalian

Mutation

G12C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer10b-EGFP-KRAS-WT

Plasmid

#164935

Purpose

Expresses EGFP cDNA and KRAS wild type cDNA in mammalian cells

Depositor

Ji Luo

Article

Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

Inserts

Enhanced green fluorescent protein
KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)

Use

Lentiviral

Promoter

Ubc Promoter and Ubc promoter

Available Since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDNA2.0 6H-TEV-KRAS

Plasmid

#111849

Purpose

Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Article

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Promoter

T5

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) K-Ras(WT)

Plasmid

#224283

Purpose

Cell transfection and expression of K-Ras

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

K-Ras (KRAS Human)

Expression

Mammalian

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode

Plasmid

#170237

Purpose

Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

G12V

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro shKRAS

Plasmid

#116871

Purpose

Tet-inducible suppression of KRAS

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

shKRAS (KRAS Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1/TO

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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