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Plasmid#77400Purpose3rd generation lentiviral gRNA plasmid targeting human CSNK2BDepositorInsertCSNK2B (CSNK2B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MINK1 gRNA (BRDN0001144839)
Plasmid#76259Purpose3rd generation lentiviral gRNA plasmid targeting human MINK1DepositorInsertMINK1 (MINK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MINK1 gRNA (BRDN0001145021)
Plasmid#76261Purpose3rd generation lentiviral gRNA plasmid targeting human MINK1DepositorInsertMINK1 (MINK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-mCherry-G12V-KRAS-IRES-Blast
Plasmid#153336PurposeLentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cellsDepositorInsertmCherry-G12V-KRAS (KRAS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVET-HA-K-RasG12V-IRES-GFP
Plasmid#107140PurposeA bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFPDepositorInsertHA-tagged Kras4B(G12V) and GFP (KRAS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhEF-1alphaAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G12V
Plasmid#111850PurposeExpresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G12V (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 12 to ValinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-mCherry-WT-KRAS-IRES-Blast
Plasmid#153335PurposeLentiviral vector plasmid for integration and constitutive expression of mCherry fused to wild-type KRAS in mammalian cellsDepositorInsertmCherry-WT-KRAS (KRAS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pInducer10b-HA-KRAS WT CO SR
Plasmid#164926PurposeExpresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRAS wild type sequenceDepositorInsertKRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
UseLentiviralTagsHA tagExpressionMutation9 Valine codons are optimized and siRNA targetin…PromoterUbc promoterAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
R703-M72-303: CMV51p> Halotag-Hs.KRAS4b
Plasmid#159679PurposeMammalian protein expression of KRAS4b-Halotag fusionDepositorInsertHalotag-Hs.KRAS4b (KRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
UseTags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
R713-M85-303: CMV51p> Halotag-Hs.KRAS4a
Plasmid#159680PurposeMammalian protein expression of KRAS4a-Halotag fusionDepositorInsertHalotag-Hs.KRAS4a (KRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pInducer10b-EGFP-KRAS G12V
Plasmid#164925PurposeExpresses EGFP cDNA and KRAS G12V cDNA in mammalian cellsDepositorInsertsUseLentiviralTagsExpressionMutationGlycine 12 changed to valine (G->T 3156)PromoterUbc Promoter and Ubc promoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-K-RAS V12
Plasmid#31200DepositorInsertK-Ras (KRAS Human)
UseGateway donor vectorTagsExpressionMutationG12V constitutively activePromoterAvailable sinceJune 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
Kras (G12V)-pcw107
Plasmid#64602Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertKRAS (KRAS Human)
UseLentiviralTagsExpressionMutationG12VPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D
Plasmid#177320PurposeLentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).DepositorInsertKRAS 4B (KRAS Human)
UseLentiviralTagsFKBP-derived dimerization domain (DD, or DmrB) an…ExpressionMammalianMutationG12DPromoterTetOn-CMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pInducer10b-HA-KRAS G12V CO SR
Plasmid#164928PurposeExpresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequenceDepositorInsertKRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
UseLentiviralTagsHA tagExpressionMutationGlycine 12 changed to valine; 9 Valine codons are…PromoterUbc promoterAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SRPK2
Plasmid#23766DepositorInsertSRPK2 (SRPK2 Human)
UseGateway donor vectorTagsExpressionMutation1MMPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G13D
Plasmid#111851PurposeExpresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G13D (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 13 to Aspartic AcidPromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-YFP-KRAS4B
Plasmid#112718PurposeExpresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.DepositorInsertYFP-KRAS4B (KRAS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
JDW 743 (pDONR221-EYFP-hKRAS4B-WT)
Plasmid#156402PurposeGateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codonDepositorInserthuman wild type KRAS4B-WT (KRAS Human)
UseGateway cloningTagsEYFPExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only