We narrowed to 441 results for: T7 rna polymerase
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Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424MutationThe conserved GDD in the NS5 polymerase gene muta…Available sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mt TEFM
Plasmid#205058PurposeTEFM protein expressionDepositorAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
C98m
Plasmid#121002PurposeMoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.DepositorInsertGp2 toxin
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-53-RtcB
Plasmid#51282PurposeE. coli expression vector for E. coli RtcB RNA ligaseDepositorInsertE. coli RtcB
Tags6xHisExpressionBacterialPromoterT7 RNA PolymeraseAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2
Plasmid#85208PurposeBackbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expressionDepositorTypeEmpty backboneUseLentiviral and RNAiExpressionMammalianPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF-BOS-T7-CA150
Plasmid#21924DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCA150 (TCERG1 Human)
TagsT7ExpressionMammalianAvailable sinceSept. 4, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDEF3-vhs
Plasmid#131696Purposeexpresses vhs in mammalian cellsDepositorPublicationInsertvhs
ExpressionMammalianPromoterCMVAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-neo-TET
Plasmid#51286Purposeexpresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassetteDepositorInsertU6 (RNU6-1 Human)
TagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianPromoterU6 upstream enhancer sequenceAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p307HU
Plasmid#27386DepositorInsertunligated form of ligase ribozyme
ExpressionBacterialAvailable sinceJan. 20, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGAL-GFP kan
Plasmid#122060Purposegalactose inducible expression of GFP in yeast. marked with URA3DepositorInsertgreen fluorescent protein
ExpressionYeastPromoterGAL1Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28C-tCorn
Plasmid#124588PurposeBacterial Expression of tRNA-Corn aptamerDepositorInserttRNA-Corn
ExpressionBacterialPromoterlacIAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Rluc-A114-N40
Plasmid#50561PurposeTemplate DNA for in vitro transcription using T7 polymeraseDepositorInsertLuciferase-A114-N40
UseCloning vectorPromoterlacAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pLKO-CMV-puro
Plasmid#131700Purposeexpresses CMV promoter driving puromycin resistanceDepositorPublicationInsertCMV-puro
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIA1385
Plasmid#166862PurposeCodon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.DepositorAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Rluc-BoxB-A114-N40
Plasmid#50562PurposeTemplate DNA for in vitro transcription using T7 polymeraseDepositorInsertLuciferase-BoxB-A114-N40
UseCloning vectorPromoterlacAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIA1363
Plasmid#166861PurposeExpression of codon optimized SARS-COV-2 Nsp8 in E. coliDepositorAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIA1362
Plasmid#166860PurposeExpression of codon optimized SARS-COV-2 Nsp7 in E. coliDepositorAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4_ZV-NS5-RdRp
Plasmid#179847PurposeThe RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89DepositorInsertZika Virus Non-structural protein 5 RdRp domain
Tags6-histineExpressionBacterialAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNG1256
Plasmid#149710PurposeExpresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complexDepositorAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only