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We narrowed to 357 results for: flpo

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Showing: 281 - 300 of 357 results

  3. Ai62(TITL-tdT) Flp-in replacement vector

    Plasmid
    #61576
    Purpose
    Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    chromatin insulator flanked TRE-LSL-tdTomato
    Use
    Recombinase-mediated cassette exchange using flp …
    Available Since
    March 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61576-plasmid-map-sequence-id-209910

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  4. MADR pDonor smFP-HA (dark) WPRE

    Plasmid
    #131007
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-HA WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    HA - 10 total HA epitope tages
    Expression
    Mammalian
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131007-plasmid-map-sequence-id-305185

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  5. MADR pDonor smFP-V5 (dark) WPRE

    Plasmid
    #131006
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    V5 - 10 total of V5 epitope tag
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131006-plasmid-map-sequence-id-305181

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  7. pCMT-flp

    Plasmid
    #67274
    Purpose
    Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis
    Depositor
    Mark Liles
    Article
    Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-
    Insert
    tetA, flp, repA101, oriR101
    Use
    Flp recombinase plasmid, temperature sensitive
    Tags
    His-SUMO (flp)
    Promoter
    tetA promoter of plasmid pACYC184 origin
    Available Since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67274-plasmid-map-sequence-id-115886

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  8. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

    Plasmid
    #131730
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    TagBFP2-V5 tag
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    131730-plasmid-map-sequence-id-318177

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  10. MADR pDonor smFP-Myc (dark) WPRE

    Plasmid
    #130987
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-Myc WPRE
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    Myc - 10 total Myc tags
    Mutation
    "dark" variant with GGG fluorophore ver…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    130987-plasmid-map-sequence-id-305000

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  11. MADR pDonor smFP-FLAG (dark) WPRE

    Plasmid
    #131005
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-FLAG WPRE
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    FLAG - 10 total FLAG tags
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131005-plasmid-map-sequence-id-305005

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  13. AAV Ef1a-EGFP-WPRE

    Plasmid
    #135428
    Purpose
    Can be used to express EGFP. Can also be used to create adeno-associated virus for delivery of the EGFP sequence
    Depositor
    Rylan Larsen
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135428-plasmid-map-sequence-id-271147

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  15. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

    Plasmid
    #220611
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-3x HA-hM3D(Gq)
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220611-plasmid-map-sequence-id-441218

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  16. AAV Ef1a-mRuby3-WPRE

    Plasmid
    #135427
    Purpose
    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.
    Depositor
    Rylan Larsen
    Insert
    mRuby3
    Use
    AAV
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135427-plasmid-map-sequence-id-271155

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  17. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid
    #220612
    Purpose
    Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220612-plasmid-map-sequence-id-441117

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  20. pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid
    #220610
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220610-plasmid-map-sequence-id-441133

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Showing: 281 - 300 of 357 results