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Plasmid Kit#1000000210PurposeToolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.DepositorAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only
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E. coli BL21 (DE3) ΔEntD
Bacterial Strain#192874PurposeDeletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.DepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
BL21(DE3) ΔserC
Bacterial Strain#197656PurposeDerivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.DepositorBacterial ResistanceNoneAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
EcAR7
Bacterial Strain#52055PurposeStrain for use in Rinehart Lab Phosphoprotein synthesis kitDepositorBacterial ResistanceNoneAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Strain: C321.ΔA
Bacterial Strain#68306PurposeGenomically recoded strain of E. coli (no genomic TAG)DepositorBacterial ResistanceBleocin (zeocin)Available sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
Z956
Bacterial Strain#200838PurposeHost strain for plasmids pYG205 and pYG215 or derivatives of the latterDepositorBacterial ResistanceNoneSpeciesEscherichia coli str. k-12 substr. mg1655Available sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry 14-3-3β ctrl
Plasmid#112031PurposeMode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)DepositorInsert14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
UseTagsSplit mCherryExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSaPIPLC His
Plasmid#173810PurposeExpression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertStaphylococcus aureus plc
UseTags6XHisExpressionBacterialMutationnonePromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-His-tagged-PSPH
Plasmid#134786PurposeStable overexpression lentiviral vector of His tagged PSPHDepositorInsertPhosphoserine phosphatase (PSPH Human)
UseLentiviralTagsHisExpressionMutationPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-BcPC-PLC
Plasmid#224255PurposeExpress N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coliDepositorUseTagsGST and HRV-3C (LEVLFQGP) siteExpressionBacterialMutationThe gene of Bacillus cereus ATCC 14579 was used f…Promoterlac promoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaBC (pCLK1210)
Plasmid#50448PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylaseDepositorInsertPPCS PPCDC
UseTags6XHIS tagExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
EcPhnD90.L1∆∆AD.L297RL301R.pRSET
Plasmid#33376Purposea single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFPDepositorInsertphnD based Phosphonate Biosensor (phnD E. coli)
UseTags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted in place of…PromoterT7Available sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
mRFP-FKBP-INPP5E(D556A)
Plasmid#183678PurposeRecruitable human type IV 5-phosphatase enzyme (catalytically inactive)DepositorInsertINPP5E (INPP5E Human)
UseTagsmRFP-FKBP12(1-108)ExpressionMammalianMutationC641A, D556APromoterCMVAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-anti-AlfaTag nanobody
Plasmid#171819PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInserttdTomato-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-anti-AlfaTag nanobody
Plasmid#171818PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertcfSGFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
SBFP2-anti-AlfaTag nanobody
Plasmid#171826PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertSBFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p15aC-4D1D-5
Plasmid#107866PurposeBacterial expression of PIS, PI4K and PI4P5KDepositorInsertsPIS
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 4-kinase β
UseTagsmycExpressionBacterialMutationPromoterproD and proD (in operon)Available sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-PLA2G15-6xHis WT
Plasmid#213603PurposeExpresses PLA2G15-6xHis in mammalian cellsDepositorInsertPhospholipase A2 Group XV (PLA2G15 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry 14-3-3β
Plasmid#111880PurposeMode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: 14-3-3β fused to C-terminal…ExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry 14-3-3σ
Plasmid#111881PurposeMode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: 14-3-3σ fused to C-terminal…ExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only