We narrowed to 8,512 results for: sgrna

1. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60226

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82703

   Purpose

   AAV backbone with a full length H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   H1/TO-L gRNA Expression Cassette Containing Tet2 gRNA
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS476-pHAGE-TO-Nme dCas9 3XGFP-SgRNA/Telomere-All-in-one

   Plasmid

   #85716

   Purpose

   Expresses dNmeCas9-3XsfGFP and telomere-targeting Nme sgRNA

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Inserts

   Nme dCas9
   telomere sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO and U6

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82705

   Purpose

   AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   U6/TO gRNA Expression Cassette Containing Tet2 gRNA
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82704

   Purpose

   AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   H1/TO-L Empty gRNA Expression Cassette
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60225

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

   Plasmid

   #218710

   Purpose

   AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

   Depositor

   Bart Nieuwenhuis

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

   Plasmid

   #168305

   Purpose

   mediated knock-in of sgRNA precursor

   Depositor

   Hui Yang

   Article

   Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

   Insert

   SaKKH-EGFP-U6-sgRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha, CBh

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pW193-lenti-sasgRNA-lacZ-pEF1s-NLS-mNeonGreen-P2A-BlastR

   Plasmid

   #170814

   Purpose

   Lentiviral vector to co-express a lacZ control sasgRNA with NLS-mNeonGreen

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Insert

   NLS-mNeonGreen-P2A-BlastR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)

    Plasmid

    #217305

    Purpose

    sgRNA BFP + Puromycin

    Depositor

    Luke Gilbert

    Insert

    pU6-sgRNA, Ef1alpha-Puro-T2A-BFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    U6, Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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