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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,418 results for: Pet2

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Showing: 2981 - 3000 of 4418 results

  5. USP7 delta C-terminus

    Plasmid
    #234074
    Purpose
    Expresses USP7 mutant in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 1-1080 (USP7 Human)
    Tags
    6xHis cleavable with thrombin
    Expression
    Bacterial
    Mutation
    E547 mutated to stop codon
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234074-plasmid-map-sequence-id-464915

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  6. pAIP4_5HFD

    Plasmid
    #234189
    Purpose
    Heterologous protein expression of disks large homolog 4 (PSD-95) (SAP90) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5HFD (Dlg4 Rat)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234189-plasmid-map-sequence-id-464154

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  7. pAIP4_4QMD

    Plasmid
    #234174
    Purpose
    Heterologous protein expression of envoplakin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4QMD (EVPL Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234174-plasmid-map-sequence-id-464139

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  9. TDRD3:JMC006-H10:C42283

    Plasmid
    #183981
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD3 in Complex with anti-TDRD3 FAB (TDRD3 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183981-plasmid-map-sequence-id-361433

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  11. 5JJW (USP15_SART3)

    Plasmid
    #101880
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15_SART3 (USP15, SART3 Human)
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101880-plasmid-map-sequence-id-194708

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  13. pJK605

    Plasmid
    #72530
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subuint (AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    72530-plasmid-map-sequence-id-141993

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  14. pJK623

    Plasmid
    #72540
    Purpose
    Produces Acetobacter aceti 1023 purSyQL associated hypothetical protein; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit (AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available Since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    72540-plasmid-map-sequence-id-132632

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  15. pJK289

    Plasmid
    #71553
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with Lys39>Ala mutant (AaAlr-K39A)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Expression
    Bacterial
    Mutation
    changes lysine-39 to alanine (please see deposito…
    Available Since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    71553-plasmid-map-sequence-id-139945

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  16. pJK501

    Plasmid
    #72246
    Purpose
    Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Gln206 to Arg and changes tryptophan-151 to pheny…
    Promoter
    T7 and endogenous
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72246-plasmid-map-sequence-id-139355

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  20. pJK579

    Plasmid
    #72426
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6), additional unique internal restriction enzyme sites
    Depositor
    T. J. Kappock
    Insert
    succinyl-CoA:acetate CoA-transferase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    4 silent mutations introduce unique internal SpeI…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72426-plasmid-map-sequence-id-133996

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Showing: 2981 - 3000 of 4418 results