Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 23,144 results for: REP

Showing: 2981 - 3000 of 23144 results

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pcDNA3.2 mir-1-1 reporter cel-mir-46

Plasmid

#46655

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-46

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter dme-let-7

Plasmid

#46662

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-let-7

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter dme-mir-34

Plasmid

#46665

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-mir-34

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-1

Plasmid

#46651

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-1

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-lsy-6

Plasmid

#46650

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-lsy-6

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-lin-4

Plasmid

#46649

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-lin-4

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter cel-let-7

Plasmid

#46648

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-let-7

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pNP2314 [Cloning backbone, Gluc/Cluc Inversion Activity Reporter]

Plasmid

#247248

Purpose

Cloning backbone for measuring bridge recombinase mediated inversion

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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10xUAS-wgSS-HA-V5-LaccID-CD2 (fly optimized, repeat fixed)

Plasmid

#234050

Purpose

expresses LaccID on the surface of fly neurons

Depositor

Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LaccID-CD2

Expression

Insect

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

Plasmid

#55217

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-twinstrep-mEGFP-SRRM2 UPR3

Plasmid

#253994

Purpose

Expressing SRRM2 undeca-amino acid repeat regions (UPR) in bacteria for protein purification

Depositor

Yongsheng Shi

Article

Yoon et al Mol Cell. 2025 Feb 6;85(3):555-570.e8. d

Insert

SRRM2 (SRRM2 Human)

Tags

10xHis and TwinStrep-mEGFP

Expression

Bacterial

Mutation

includes UPR3 only (aa 645-677)

Promoter

T7

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-twinstrep-RBBP6 NTD+IDR4

Plasmid

#253992

Purpose

Expressing RBBP6 variants in bacteria for protein purification

Depositor

Yongsheng Shi

Article

Yoon et al Mol Cell. 2025 Feb 6;85(3):555-570.e8. d

Insert

RBBP6 (RBBP6 Human)

Tags

10xHis and TwinStrep

Expression

Bacterial

Mutation

includes NTD fused to IDR4 only (aa 351-1681 dele…

Promoter

T7

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-CLIP-1XTPR-SNAP-StrepTag

Plasmid

#248178

Purpose

For the mammalian expression of FLAG-CLIP-1XTPR-SNAP-StrepTag fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2026.03.05.709886

Insert

FLAG-CLIP-1XTPR-SNAP-StrepTag

Use

1377

Tags

FLAG, CLIP and SNAP, StrepTag

Expression

Mammalian

Mutation

WT

Promoter

CMV & T7

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-CLIP-2XTPR-SNAP-StrepTag

Plasmid

#248179

Purpose

For the mammalian expression of FLAG-CLIP-2XTPR-SNAP-StrepTag fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2026.03.05.709886

Insert

FLAG-CLIP-2XTPR-SNAP-StrepTag

Use

1479

Tags

FLAG, CLIP and SNAP, StrepTag

Expression

Mammalian

Mutation

WT

Promoter

CMV & T7

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-CLIP-3XTPR-SNAP-StrepTag

Plasmid

#248180

Purpose

For the mammalian expression of FLAG-CLIP-3XTPR-SNAP-StrepTag fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2026.03.05.709886

Insert

FLAG-CLIP-3XTPR-SNAP-StrepTag

Use

1581

Tags

FLAG, CLIP and SNAP, StrepTag

Expression

Mammalian

Mutation

WT

Promoter

CMV & T7

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pRESTA-FLAG-CLIP-1XTPR-SNAP-StrepTag

Plasmid

#248182

Purpose

For the bacterial expression of FLAG-CLIP-1XTPR-SNAP-StrepTag fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2026.03.05.709886

Insert

FLAG-CLIP-1XTPR-SNAP-StrepTag

Use

1374

Tags

HisTag, FLAG, CLIP and SNAP, StrepTag

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pRESTA-FLAG-CLIP-2XTPR-SNAP-StrepTag

Plasmid

#248183

Purpose

For the bacterial expression of FLAG-CLIP-2XTPR-SNAP-StrepTag fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2026.03.05.709886

Insert

FLAG-CLIP-2XTPR-SNAP-StrepTag

Use

1476

Tags

HisTag, FLAG, CLIP and SNAP, StrepTag

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pRESTA-FLAG-CLIP-3XTPR-SNAP-StrepTag

Plasmid

#248184

Purpose

For the bacterial expression of FLAG-CLIP-3XTPR-SNAP-StrepTag fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2026.03.05.709886

Insert

FLAG-CLIP-3XTPR-SNAP-StrepTag

Use

1578

Tags

HisTag, FLAG, CLIP and SNAP, StrepTag

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-FLAG-CLIP(Q149TAG)-SNAP-StrepTag

Plasmid

#248185

Purpose

For the mammalian expression and non-cannonical labelling of FLAG-CLIP(Q149TAG)-SNAP-StrepTag fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2026.03.05.709886

Insert

FLAG-CLIP(Q149TAG)-SNAP-StrepTag

Use

1257

Tags

FLAG, CLIP and SNAP, StrepTag

Expression

Mammalian

Mutation

Q149TAG

Promoter

CMV & T7

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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P1834: Eva3_RHISA-P1142_AMBCA-P991_AMCA-StrepII-8xHis

Plasmid

#249612

Purpose

Expresses Strep and His-tagged Eva3_RHISA-P1142_AMBCA-P991_AMCA in mammalian cells

Depositor

Shoumo Bhattacharya

Insert

EVA3_RHISA:E1142_AMBCJ:EV991_AMBCJ (Genetic fusion of Evasin proteins EVA3, E1142 and EV991 with G4S linkers)

Use

Mammalian expression vector, with ampicillin resi…

Tags

Strep II, 8xHis

Expression

Mammalian

Promoter

CMV enhancer-chicken beta-actin promoter

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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Showing: 2981 - 3000 of 23144 results

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