We narrowed to 8,345 results for: construct

1. AAV-6P-SEW-YC3.6

   Plasmid

   #31379

   Depositor

   Fritjof Helmchen

   Article

   Minderer et al J Physiol. 2011 Nov 14.

   Insert

   Yellow Cameleon 3.60
   

   Use

   AAV

   Tags

   Venus and eCFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-P2A-mKate2 no-pA

   Plasmid

   #80828

   Purpose

   P2A followed by mKate2 without pA

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   p3E-P2A-mKate2 no pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMycoFos

   Plasmid

   #84577

   Purpose

   Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.

   Depositor

   Nicholas Coleman

   Article

   Ly et al J Microbiol Methods. 2011 Sep;86(3):320-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pBS-ermGFP

   Plasmid

   #27172

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   Available since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBYK01

   Plasmid

   #138656

   Purpose

   phiOZJ integrating Streptomyces expression vector lacking RDF

   Depositor

   Joshua Blodgett

   Article

   Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PermE* variant sequence from pDA1652

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYZ146

   Plasmid

   #98406

   Purpose

   Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Insert

   gRNA targeting Sp.leu1 (leu1 Fission Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJMD5

   Plasmid

   #138652

   Purpose

   phiBT1 integrating Streptomyces expression vector

   Depositor

   Joshua Blodgett

   Article

   Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PermE* variant sequence from pDA1652

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYZ164

   Plasmid

   #98408

   Purpose

   Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Insert

   gRNA targeting Sp.his3 (his3 Fission Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAMP-CY1_NF1 T1 (+minintr, KDR)

   Plasmid

   #119200

   Purpose

   transfer vector

   Depositor

   Helen Morrison

   Article

   Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

   Insert

   NF1 type 1 mini-gene (NF1 Human)
   

   Use

   Transfer vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJMD13

    Plasmid

    #138653

    Purpose

    phiC31 integrating Streptomyces expression vector

    Depositor

    Joshua Blodgett

    Article

    Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PermE* variant sequence from pDA1652

    Available since

    June 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-EF1a-SOX2

    Plasmid

    #35388

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    TALE-Sox2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

12. p5E-CMVmin

    Plasmid

    #80802

    Purpose

    minimal cytomegalovirus immediate early enhancer/promoter (CMVmin) from pcDNA3

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    minimal cytomegalovirus immediate early enhancer/promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSET FLIPglu-600uDelta13

    Plasmid

    #13559

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A Delta13
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    F16A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. LI F-G Spec

    Plasmid

    #104521

    Purpose

    LI resistance gene module

    Depositor

    Thomas Lahaye

    Article

    Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

    Insert

    Spectinomycin resistance gene
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CH-GECO2.1

    Plasmid

    #52099

    Purpose

    mCherry-based Calcium indicators

    Depositor

    Robert Campbell

    Article

    Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

    Insert

    CH-GECO2.1
    

    Use

    Tags

    6 His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD promotor

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pJMD14

    Plasmid

    #138654

    Purpose

    phiOZJ integrating Streptomyces expression vector, intact RDF

    Depositor

    Joshua Blodgett

    Article

    Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PermE* variant sequence from pDA1652

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOGG010

    Plasmid

    #113984

    Purpose

    Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    RK2 (IncP)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSG81-pLas-tetO-deGFPssrA

    Plasmid

    #102454

    Purpose

    Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    deGFP
    

    Use

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    Promoter

    pLas-tetO

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mUAVbla:tetR 5′

    Plasmid

    #149379

    Purpose

    mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

    Depositor

    Mark Pallen

    Article

    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

    Insert

    tetA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEpic

    Plasmid

    #84372

    Purpose

    3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance.

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge