We narrowed to 17,425 results for: emb

1. pCW57-CMV-MAAP9

   Plasmid

   #218246

   Purpose

   1) Ectopic expression of MAAP9 protein in mammalian cells. 2) Packaging MAAP9 sequence into lentivirus for stable expression in mammalian cells.

   Depositor

   David Schaffer

   Article

   Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

   Insert

   Membrane-Associated Accessory Protein from Adeno-Associated Virus 9 driven by CMV promoter
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-myr-mTagBFP2-Flag (JDW 1183)

   Plasmid

   #224492

   Purpose

   Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   myr-mTagBFP2-Flag
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMV_AA091

   Plasmid

   #216106

   Purpose

   CRISPRa, gRNA expression with modified trRNA to recruit activators via PP7 tags (guide only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   gRNA expression with modified trRNA to recruit activators via PP7 tags
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR4 CDS A. thaliana MAMI

   Plasmid

   #209186

   Purpose

   pENTR4 plasmid with CDS of Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI, AT5G54110.1) including stop codon for N-terminal epitope tags

   Depositor

   Lennart Wirthmueller

   Article

   Simranjit et al bioRxiv 2023.09.25.558804

   Insert

   AT5G54110.1 (MAMI Mustard Weed)
   

   Use

   Gateway-compatible entry vector

   Promoter

   none

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMX1463

   Plasmid

   #208805

   Purpose

   Expresses plasma-membrane-bound FRB-SBFP2 for rapid relocalization of cytoplasmic proteins tagged with FKBP upon addition of rapamycin

   Depositor

   Linda Wordeman

   Article

   Wordeman et al Mol Biol Cell. 2016 Apr 15;27(8):1300-9.

   Insert

   FRB (CRP Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. FKBP12-CB2mutx2-mTagBFP2-HAx2

   Plasmid

   #207147

   Purpose

   Tandem mutant calcium-buffer with rapalog dimerizer

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   Calbindin-2 (CALB2 Human)
   

   Tags

   FKBP12, HA, and mTagBFP2

   Expression

   Mammalian

   Mutation

   E40Q, E87Q, E131Q, E175Q, E219Q

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-mini v2-HF24_F4

   Plasmid

   #195718

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#4 of 24 fragment splits in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   March 20, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pUC57-mini v2-HF24_F20

   Plasmid

   #195734

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#20 of 24 fragment splits in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   March 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pUC57-mini v2-HF24_F5

   Plasmid

   #195719

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#5 of 24 fragment splits in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   March 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pUC57-mini v2-HF24_F12

    Plasmid

    #195726

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#12 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAc5.1B-EGFP-DmNanos-del50-236_AC

    Plasmid

    #148459

    Purpose

    Insect Expression of DmNanos-del50-236

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmNanos-del50-236 (nos Fly)
    

    Expression

    Insect

    Mutation

    sequence contains a deletion of N283 and a mutati…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1B-lambdaN-HA-DmNot1_1710-2505-V2002E-dsRNAres_AC

    Plasmid

    #148463

    Purpose

    Insect Expression of DmNot1_1710-2505-V200E-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmNot1_1710-2505-V200E-dsRNAres (Not1 Fly)
    

    Expression

    Insect

    Mutation

    4 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-lambdaN-HA-DmNot1_1710-2505-H2067D-dsRNAres_AD

    Plasmid

    #148500

    Purpose

    Insect Expression of DmNot1_1710-2505-H2067D-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmNot1_1710-2505-H2067D-dsRNAres (Not1 Fly)
    

    Expression

    Insect

    Mutation

    4 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-HA-DmMe31B-QHTKtoAAAA-dsRNAres_AC

    Plasmid

    #148391

    Purpose

    Insect Expression of DmMe31B-QHTKtoAAAA-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    DmMe31B-QHTKtoAAAA-dsRNAres (me31B Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation A1101G compared to the sequen…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAc5.1B-HA-DmMe31B-R307AK313AK314A-dsRNAres_AC

    Plasmid

    #148392

    Purpose

    Insect Expression of DmMe31B-R307AK313AK314A

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    DmMe31B-R307AK313AK314A (me31B Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation A1101G compared to the sequen…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc5.1B-HA-DmMe31B-RFQNRtoEDAFA-dsRNAres_AC

    Plasmid

    #148393

    Purpose

    Insect Expression of DmMe31B-RFQNRtoEDAFA-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    DmMe31B-RFQNRtoEDAFA-dsRNAres (me31B Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation A1101G compared to the sequen…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-lambdaN-HA-DmMe31B-QHTKRKKtoAAAAAAA_AC

    Plasmid

    #148396

    Purpose

    Insect Expression of DmMe31B-QHTKRKKtoAAAAAAA

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    DmMe31B-QHTKRKKtoAAAAAAA (me31B Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation A1101G compared to the sequen…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1B-lambdaN-HA-DmMe31B-R307AK313AK314A_AC

    Plasmid

    #148397

    Purpose

    Insect Expression of DmMe31B-R307AK313AK314A

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    DmMe31B-R307AK313AK314A (me31B Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation A1101G compared to the sequen…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-DmNot1_1147-1673_AC

    Plasmid

    #148398

    Purpose

    Insect Expression of DmNot1_1147-1673

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    DmNot1_1147-1673 (Not1 Fly)
    

    Expression

    Insect

    Mutation

    2 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-lambdaN-HA-DmNot1_1149-1962-dsRNAres_AC

    Plasmid

    #148399

    Purpose

    Insect Expression of DmNot1_1149-1962-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    DmNot1_1149-1962-dsRNAres (Not1 Fly)
    

    Expression

    Insect

    Mutation

    4 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge