We narrowed to 4,191 results for: Pet2

1. pJK325

   Plasmid

   #71556

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes alanine-367 to aspartate

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJK500

   Plasmid

   #72245

   Purpose

   Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   T. J. Kappock

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

   Promoter

   T7 and endogenous

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK345

   Plasmid

   #71559

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   deletes alanine-367 and alanine-368

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJK344

   Plasmid

   #71558

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   deletes alanine-367 (same as deleting alanine-368)

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK624

   Plasmid

   #72539

   Purpose

   Produces Acetobacter aceti 1023 OrfY; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit with C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6)

   Depositor

   T. J. Kappock

   Inserts

   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK625

   Plasmid

   #72541

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); glutaminase subunit (AaPurQ); large subunit (AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK392

   Plasmid

   #70236

   Purpose

   Protein production from a synthetic gene encoding Treponema denticola ATCC 35405 PurE-H40N

   Depositor

   T. J. Kappock

   Article

   Tranchimand et al Biochemistry. 2011 May 31;50(21):4623-37

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   His40Asn

   Promoter

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 4QQI

   Plasmid

   #61949

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ANKRA:JMC023-D07:C211743 (ANKRA2 Human)
   

   Use

   Tags

   6xHis and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   March 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. 2MJ8

   Plasmid

   #61956

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CDYL2 (CDYL2 Human)
   

   Use

   Tags

   6xHis and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   March 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. 4LG6

    Plasmid

    #60093

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ANKRA:JMC023-D07:C211743 (ANKRA2 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

11. L3MBTL1 (3P8H)

    Plasmid

    #28265

    Depositor

    Cheryl Arrowsmith

    Insert

    L3MBTL1 (L3MBTL1 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acid residues 200-530.

    Promoter

    Available since

    April 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

12. CHKB (2IG7)

    Plasmid

    #25516

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CHKB (CHKB Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. ARL10B

    Plasmid

    #25522

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ARL10B (ARL8A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. CENTA1 (3LJU)

    Plasmid

    #25340

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CENTA1:K3-F370 (ADAP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids K3-F370

    Promoter

    Available since

    Aug. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. CENTA1 (3FEH)

    Plasmid

    #25262

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CENTA1:K3-F370 (ADAP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids K3-F370

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. WDR5 (2H9O)

    Plasmid

    #25230

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5_01 (WDR5 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. PRRX2-pET

    Plasmid

    #21011

    Depositor

    Corey Largman

    Article

    Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

    Insert

    PRRX2 (Prrx2 Mouse)
    

    Use

    Tags

    His and T7-tag

    Expression

    Bacterial

    Mutation

    Missing first 3 N-terminal amino acids

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

18. FKBP8

    Plasmid

    #25609

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    FKBP8 (FKBP8 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    FKBP-type PPIase Domain, residues 90-205

    Promoter

    Available since

    Aug. 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. Human USP30 (64-502, construct 2, MG-26-18)

    Plasmid

    #110744

    Purpose

    Bacterial expression of human USP30 (construct 2)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-3C-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PT7lacO-RF1-His6

    Plasmid

    #224372

    Purpose

    Express RF1 protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    RF1 (prfA E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge