We narrowed to 8,345 results for: construct

1. pOGG011

   Plasmid

   #113985

   Purpose

   Vector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectors

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   pBBR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-EF1a-TALE1

   Plasmid

   #27969

   Depositor

   Feng Zhang

   Article

   Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

   Insert

   TALE1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4092694

   Plasmid

   #153907

   Purpose

   Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | t7 consensus from NEB, G is +1

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. p3E-P2A-CFP no-pA

   Plasmid

   #80827

   Purpose

   P2A followed by CFP without pA

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   p3E-P2A-CFP no pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG306-pTet07-synYFP[TTG/AGA]-nLuc

   Plasmid

   #199756

   Purpose

   Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling

   Depositor

   Brian Zid

   Article

   Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

   Insert

   synYFP[TTG/AGA]-nLuc
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TetO7

   Available since

   May 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAMP-CY1_NF1 T2 (+minintr, KDR)

   Plasmid

   #119201

   Purpose

   transfer vector

   Depositor

   Helen Morrison

   Article

   Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

   Insert

   NF1 type 2 mini-gene (NF1 Human)
   

   Use

   Transfer vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNH034

   Plasmid

   #42150

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   RT-cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LV 1-5

   Plasmid

   #68411

   Purpose

   Lentiviral backbone for use with GMAP

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Type

   Empty backbone

   Use

   Lentiviral; Gmap

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOGG012

   Plasmid

   #113986

   Purpose

   Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Partition genes (parABCDE)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMK1200

    Plasmid

    #84219

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherry

    Depositor

    Martin Kampmann, Jonathan Weissman

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFGC5941-PacI

    Plasmid

    #44020

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-H1-SGIPZ_NF1 sh1miR

    Plasmid

    #119206

    Purpose

    knockdown of NF1

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    sh1miR targeting NF1 (NF1 Human, Rat, Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBlue-TAL

    Plasmid

    #49401

    Purpose

    The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

    Depositor

    Michal Zurovec

    Article

    Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046538

    Plasmid

    #149128

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. p3E-GFP no-pA

    Plasmid

    #80813

    Purpose

    GFP without pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    GFP no pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFP

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBW-m4137387

    Plasmid

    #149544

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt;19-aa Ctrunc

    Promoter

    CMV

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

17. LI F-G Kan

    Plasmid

    #104523

    Purpose

    LI resistance gene module

    Depositor

    Thomas Lahaye

    Article

    Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

    Insert

    Kanamycin resistance gene
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3E-CMVmin:nlsGFP pA

    Plasmid

    #80821

    Purpose

    CMVmin-promoted nlsGFP with pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    p3E-CMV-nlsGFP pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVmin

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGBW-m4046494

    Plasmid

    #149137

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 surface glycoprotein (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pAMP-CY1_NF1 T1 R1276P (+minintr, KDR)

    Plasmid

    #119202

    Purpose

    transfer vector

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    NF1 type 1 mini-gene (NF1 Human)
    

    Use

    Transfer vector

    Tags

    Expression

    Mutation

    R1276P

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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