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Showing: 3001 - 3020 of 7226 results

  5. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174647
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    CMV (with TetOn)
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174647-plasmid-map-sequence-id-345296

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  14. pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid
    #165492
    Purpose
    Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity
    Depositor
    Thomas Oertner
    Article
    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10
    Insert
    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    Use
    AAV
    Tags
    darkVenus
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1
    Available since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165492-plasmid-map-sequence-id-330311

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  19. pB80-KIF1A(1-383)-GFP-SSPB(nano)

    Plasmid
    #174628
    Purpose
    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(nano)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-383)-GFP-SSPB(nano) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(nano)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del;
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174628-plasmid-map-sequence-id-346988

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  20. pB80-KIF1A(1-365)-GFP-SSPB(micro)

    Plasmid
    #174629
    Purpose
    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174629-plasmid-map-sequence-id-346983

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Showing: 3001 - 3020 of 7226 results