We narrowed to 3,466 results for: ttl

1. pENTR2b ITGA2-tail-YPet (GFFKR/GAAKR)

   Plasmid

   #145144

   Purpose

   Shuttle vector with YPet-tagged ITGA2 cytoplasmic tail as an insert with a GFFKR/GAAKR mutation

   Depositor

   Johanna Ivaska

   Article

   Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

   Insert

   ITGA2-tail-YPet (GFFKR/GAAKR) (ITGA2 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

   Plasmid

   #228400

   Purpose

   Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

   Depositor

   Dirk Daelemans

   Article

   Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

   Insert

   NES/PKI-mNeonGreen2x-NLS/FUS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro

   Plasmid

   #228398

   Purpose

   Encodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasm

   Depositor

   Dirk Daelemans

   Article

   Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

   Insert

   NLS/cMyc-AcGFP2x-NES/iKB2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro

   Plasmid

   #228399

   Purpose

   Encodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal PKI NES that shuttles between the nucleus and the cytoplasm

   Depositor

   Dirk Daelemans

   Article

   Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

   Insert

   3xFLAG-NLS/SV40-AcGFP-NES/PKI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYPQ265E7

   Plasmid

   #213470

   Purpose

   Gateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

   Depositor

   Yiping Qi

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Insert

   TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI
   

   Use

   CRISPR; Gateway compatible tada-cdd-32aa linker-z…

   Tags

   No

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYPQ265E8

   Plasmid

   #213471

   Purpose

   Gateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

   Depositor

   Yiping Qi

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Insert

   TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI
   

   Use

   CRISPR; Gateway compatible tada-cdd_v106w-32aa li…

   Tags

   No

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)

   Plasmid

   #191631

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadS

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   Dopamine dehydroxylase (R506S mutation, inactive form)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)

   Plasmid

   #191651

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. attB53_SNCB-_attB53

   Plasmid

   #183615

   Purpose

   RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Inserts

   LaG17 GFP nanobody SynNotch tTA
   TRE-mCherry-SV40pA
   tagBFP
   

   Use

   Rmce

   Tags

   Myc and human CD8a signal sequence

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221 CARD8 FIIND F405G + S297A

    Plasmid

    #169974

    Purpose

    pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    S297A, F405G

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR221 CARD8 FIIND E274R + S297A

    Plasmid

    #169968

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    S297A, E274R

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR221 CARD8 FIIND R394E + S297A

    Plasmid

    #169972

    Purpose

    pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    S297A, R394E

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221 CARD8 PAM E274R

    Plasmid

    #169977

    Purpose

    pDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    PAM, E274R

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221 NLRP1 delta787-843

    Plasmid

    #166821

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation _787-843 (Zhong et al., 2016) (no stop codon)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    _787-843

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221 Ub-NLRP1 S1213 P1214R

    Plasmid

    #166830

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    P1214R

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221 NLRP1 T755N

    Plasmid

    #166820

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    T755N

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221 NLRP1 R726W

    Plasmid

    #166819

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation R726W (Grandemange et al., 2017) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    R726W

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

    Plasmid

    #149276

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Promoter

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIA33

    Plasmid

    #120803

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

    Depositor

    David S. Weiss, Craig Ellermeier

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Inserts

    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Base-pairing region targets red fluorescent prote…

    Promoter

    Pgdh and Pxyl

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCK306

    Plasmid

    #110544

    Purpose

    rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

    Inserts

    PrhaBAD
    yfp
    rhaS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A, PrhaBAD, and kanR promoter (upstream of kanR…

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge