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pUBQ10:WNFLS2-1

Plasmid

#202189

Purpose

Express 'Washington navel' Orange FLS2-1 gene under the UBQ10 promoter

Depositor

Gitta Coaker

Article

Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

Insert

Flagellin sensing 2

Tags

HA

Expression

Plant

Promoter

AtUBQ10

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pUBQ10:LLFLS2-1

Plasmid

#202187

Purpose

Express 'Frost Lisbon' Lemon FLS2-1 gene under the UBQ10 promoter

Depositor

Gitta Coaker

Article

Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

Insert

Flagellin sensing 2

Tags

HA

Expression

Plant

Promoter

AtUBQ10

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BLRF1

Plasmid

#195771

Purpose

Gateway Entry Clone of Epstein-Barr virus BLRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BLRF1

Use

Gateway entry vector

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-luciferase

Plasmid

#198590

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

luciferase

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BdRF1

Plasmid

#195802

Purpose

Gateway Entry Clone of Epstein-Barr virus BdRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BdRF1

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BBRF3

Plasmid

#195808

Purpose

Gateway Entry Clone of Epstein-Barr virus BBRF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BBRF3

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BDLF2

Plasmid

#195809

Purpose

Gateway Entry Clone of Epstein-Barr virus BDLF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BDLF2

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-SM

Plasmid

#195814

Purpose

Gateway Entry Clone of Epstein-Barr virus SM

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

SM

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BFRF0.5

Plasmid

#195772

Purpose

Gateway Entry Clone of Epstein-Barr virus BFRF0.5

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BFRF0.5

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BLLF2

Plasmid

#195774

Purpose

Gateway Entry Clone of Epstein-Barr virus BLLF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BLLF2

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BDLF3

Plasmid

#195786

Purpose

Gateway Entry Clone of Epstein-Barr virus BDLF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BDLF3

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BBRF2

Plasmid

#195792

Purpose

Gateway Entry Clone of Epstein-Barr virus BBRF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BBRF2

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BLLF3

Plasmid

#195793

Purpose

Gateway Entry Clone of Epstein-Barr virus BLLF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BLLF3

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BDLF1

Plasmid

#195794

Purpose

Gateway Entry Clone of Epstein-Barr virus BDLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BDLF1

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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Sha3Cas9

Plasmid

#192136

Purpose

Expresses Sha3Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

Sha3Cas9

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No8

Plasmid

#194902

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-8 targeting the promoter of human MECP2.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.8

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No6

Plasmid

#194900

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-6 targeting the promoter of human MECP2.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.6

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No7

Plasmid

#194901

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-7 targeting the promoter of human MECP2.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.7

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLY237

Plasmid

#184150

Purpose

Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 1)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-R1

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLY251

Plasmid

#184153

Purpose

Reprogrammed short tracrRNA for hijacking mRNA of arsenic responsive gene cluster (site 2)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-Ar2

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Immune checkpoint (library 2) Backbone

Plasmid

#192363

Purpose

Plasmid vector for in vivo immune checkpoint screening library (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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T cell activation (library 1) Backbone

Plasmid

#192364

Purpose

Plasmid vector for in vitro Jurkat activation screening (for immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Gly-tRNA-gRNA scaffold for library 2

Plasmid

#192365

Purpose

tRNA sequence provider (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gly tRNA-gRNA scaffold

Use

For golden gate assembly

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Pro-tRNA-gRNA scaffold for library 2

Plasmid

#192366

Purpose

tRNA sequence provider (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Pro tRNA-gRNA scaffold

Use

For golden gate assembly

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Gly-tRNA-gRNA scaffold for library 1

Plasmid

#192368

Purpose

tRNA sequence provider (for immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gly tRNA-gRNA scaffold

Use

For golden gate assembly

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Pro-tRNA-gRNA scaffold for library 1

Plasmid

#192369

Purpose

tRNA sequence provider (for immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Pro tRNA-gRNA scaffold

Use

For golden gate assembly

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1127B

Plasmid

#190997

Purpose

Expresses arrays of gRNA targeting rel1 promoter under U6b/c promoter

Depositor

Omar Akbari

Article

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

U6b:sgRNArel1A1 - U6c:sgRNArel1A2 - U6b:sgRNArel1A3 - U6c:sgRNArel1A4

Expression

Insect

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265K

Plasmid

#173997

Purpose

Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

UNG-rAPOBEC1(R33A)-zCas9(D10A)

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

R33A in rAPOBEC1 and D10A in zCas9

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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PlmdCas12e VPR

Plasmid

#188516

Purpose

Expresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-VPR

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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px459-H3.3 (H3f3b) sgRNA

Plasmid

#186931

Purpose

H3f3b sgRNA used for genomic targeting at H3f3b C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

H3f3b sgRNA (H3f3b Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Daxx KO sgRNA

Plasmid

#186939

Purpose

Daxx KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Daxx KO sgRNA (Daxx Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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WDR4_pcDNA6.2/EmGFP-Bsd

Plasmid

#176984

Purpose

Mammalian expression vector encoding WDR4 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

WDR4 (WDR4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3137

Plasmid

#179617

Purpose

Punc-4::GCaMP6::RFP unc-54 3' UTR C.elegans A-MNs and others expression of GCaMP6::RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

GCaMP6

Tags

RFP

Expression

Worm

Promoter

Punc-4

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSMART-Ala2

Plasmid

#71326

Purpose

NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

insulated waaHp from E. coli

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pPRIChp-hOtub1

Plasmid

#174743

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Article

Zhou et al Nat Immunol. 2019 Jul;20(7):879-889. doi

Insert

OTUB1 (OTUB1 Human)

Use

Retroviral

Tags

HA

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-zfCSF3a

Plasmid

#168201

Purpose

Express zebrafish Csf3a, mRNA synthesis

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

Insert

Csf3a (csf3a Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Mutation

Deletion of A27

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa-dsRBD(+6)

Plasmid

#171548

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Staufen-dsRBD(+6)

Plasmid

#171546

Purpose

Encoding part of the Drosophila Staufen protein from M571 to P656

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Staufen (stau Fly)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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PW124-LMNA 5'HA-TriTag (mCherry)-3'HA (HDR donor)

Plasmid

#164047

Purpose

CRISPR donor plasmid to insert TriTag (mCherry) into the N-terminus of human LMNA gene

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

mCherry-TriTag

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-5U6-sgRNAs-hsyn-EGFP

Plasmid

#112213

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

sgRNAs

Use

AAV

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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