We narrowed to 32,111 results for: Eng

1. pCAGGS 3xFLAG Mbnl2

   Plasmid

   #217032

   Purpose

   Overexpression of Mbnl2 in mammalian cells

   Depositor

   Chaolin Zhang

   Article

   Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

   Insert

   Mbnl2 (Mbnl2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDisplay-GRAB_SST1.0

   Plasmid

   #208664

   Purpose

   Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE-GTF2IRD1__ALK

   Plasmid

   #116821

   Purpose

   Lentiviral expression of GTF2IRD1__ALK fusion

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   GTF2IRD1__ALK
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSEMS-Tom20-mEGFP-reHaloTagF

   Plasmid

   #200190

   Purpose

   Tom20 with reversible HaloTagF variant for fluorescent labeling

   Depositor

   Jacob Piehler

   Article

   Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

   Insert

   Tom20 (TOMM20 Human)
   

   Use

   Tags

   mEGFP and reHaloTagF

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAcBac1-EcTrp-TGA

   Plasmid

   #228780

   Purpose

   aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTrpRS
   EcWtR
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TGA suppressor

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

   Plasmid

   #164471

   Purpose

   Expresses sgRNA targeting human GJA1 locus

   Depositor

   Markus Kuehn

   Article

   Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

   Insert

   Gap junction alpha-1 (GJA1) (GJA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. OCT4-eGFP-2A-Puro

   Plasmid

   #31939

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

   Inserts

   OCT4 (POU5F1 Human)
   eGFP (eGFP )
   

   Use

   Targeting donor

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hSyn-GRAB_SST1.0

   Plasmid

   #208665

   Purpose

   Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSW031

   Plasmid

   #231144

   Purpose

   Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.

   Depositor

   Philip Cole

   Article

   Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021

   Insert

   S. aureus Sortase A mutant
   

   Use

   Tags

   Nostoc punctiform split intein C-terminal fragmen…

   Expression

   Bacterial

   Mutation

   P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…

   Promoter

   T7

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn-GRAB_UCN1.0

    Plasmid

    #208669

    Purpose

    Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDisplay-GRAB_CRF1.0

    Plasmid

    #208655

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. OCT4-eGFP-PGK-Puro

    Plasmid

    #31937

    Purpose

    Oct4 targeting donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CMV-VPR-S-L1-dCjCas9 MiniCAFE

    Plasmid

    #169910

    Purpose

    Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    MiniCAFE
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D8A, HNH-truncation (Δ495–609 aa) in CjCas9

    Promoter

    CMV

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE-ALDH5A1__MAP3K5

    Plasmid

    #116807

    Purpose

    Lentiviral expression of ALDH5A1__MAP3K5 fusion

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ALDH5A1__MAP3K5
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYTSK01K_0G7

    Plasmid

    #177296

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

    Depositor

    Karl-Erich Jaeger

    Article

    Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

    Insert

    PaacC1-aacC1
    

    Use

    Synthetic Biology; Yeast expression, tn7 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDisplay-GRAB_UCN1.0

    Plasmid

    #208667

    Purpose

    Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hSyn-GRAB_NPY1.0

    Plasmid

    #208676

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDisplay-GRAB_NTS1.0

    Plasmid

    #208670

    Purpose

    Expresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mEGFP-N1-LATS2

    Plasmid

    #166474

    Purpose

    Expresses fusion of mEGFP and LATS2

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, LATS2 (LATS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDisplay-EGFP-CAAX

    Plasmid

    #208683

    Purpose

    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge