We narrowed to 32,558 results for: Eng

1. pAAV-syn-FLEX-tTA

   Plasmid

   #178516

   Purpose

   AAV vector for Cre recombinase dependent tetracycline-controlled transactivator (tTA) expression

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   tTA
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28 mePOU-POU

   Plasmid

   #206392

   Purpose

   Expresses the DNA binding domain of the ePOU with a 6xHis tag for protein purification

   Depositor

   Ralf Jauch

   Article

   Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

   Insert

   ePOU
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   POU DNA binding domain only

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. iCAP-BI-49-KanR

   Plasmid

   #203534

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   AAV-BI49 Cap
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. iCAP-BI-65-KanR

   Plasmid

   #203536

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   AAV-BI65 Cap
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIALD2HMGr

   Plasmid

   #185867

   Purpose

   Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETDuet1-LanCL2-H264A-C-Ter-His

   Plasmid

   #154187

   Purpose

   To express LanCL2-H264A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   His264->Ala264

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-15b-LanCL1-H219A

   Plasmid

   #154190

   Purpose

   To express LanCL1-H219A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL1
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   His219->Ala219

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-15b-LanCL1-C322A

   Plasmid

   #154191

   Purpose

   To express LanCL1-C322A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL1
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Cys322->Ala322

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMAL-p2x-LanCL2

   Plasmid

   #154192

   Purpose

   To express MBP-LanCL2 in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Tags

   Maltose binding protein (MBP)

   Expression

   Bacterial

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-TTBG

    Plasmid

    #115232

    Purpose

    Lentiviral transfer plasmid for expression of SAD B19 G and mTagBFP2

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    mTagBFP2 and rabies virus SAD B19 strain glycoprotein gene (B19G)
    

    Expression

    Mammalian

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-TTBL

    Plasmid

    #115233

    Purpose

    Lentiviral transfer plasmid for expression of SAD B19 L and mTagBFP2

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    mTagBFP2 and rabies virus SAD B19 strain polymerase gene (B19L)
    

    Expression

    Mammalian

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PLKO.1-Cd155 shRNA2

    Plasmid

    #166488

    Purpose

    Mouse Cd155 RNAi

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    shRNA for mouse Cd155 (Pvr Mouse)
    

    Use

    Lentiviral

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PLKO.1-Cd155 shRNA1

    Plasmid

    #166487

    Purpose

    Mouse Cd155 RNAi

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    shRNA for mouse Cd155 (Pvr Mouse)
    

    Use

    Lentiviral

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCherry-N1-MOB1

    Plasmid

    #166477

    Purpose

    Expresses fusion of mCherry and MOB1

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mCherry, MOB1 (MOB1A Human)
    

    Expression

    Mammalian

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PatoM-OG241

    Plasmid

    #72842

    Purpose

    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoM
    

    Expression

    Bacterial

    Promoter

    PatoM

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEXP5-NT-7th R

    Plasmid

    #42573

    Depositor

    Ivan Korendovych

    Article

    Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

    Insert

    mutated calmodulin gene
    

    Tags

    Polyhistidine (6×His) region, TEV recognition sit…

    Expression

    Bacterial

    Mutation

    changed Phenylalanine 92 to Glutamine acid, Isole…

    Promoter

    T7

    Available Since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

    Plasmid

    #168305

    Purpose

    mediated knock-in of sgRNA precursor

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    SaKKH-EGFP-U6-sgRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1alpha, CBh

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn-GRAB_CRF1.0

    Plasmid

    #208658

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDisplay-GRAB_CRF1.0

    Plasmid

    #208655

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDisplay-EGFP-CAAX

    Plasmid

    #208683

    Purpose

    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge