We narrowed to 32,558 results for: Eng

1. pCMV_iPE-C_P2A_Csy4

   Plasmid

   #232428

   Purpose

   Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   iPE-C–P2A–Csy4
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDisplay-GRAB_SST1.0

   Plasmid

   #208664

   Purpose

   Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSW031

   Plasmid

   #231144

   Purpose

   Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.

   Depositor

   Philip Cole

   Article

   Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021

   Insert

   S. aureus Sortase A mutant
   

   Tags

   Nostoc punctiform split intein C-terminal fragmen…

   Expression

   Bacterial

   Mutation

   P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…

   Promoter

   T7

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV_Cas9–superNLS

   Plasmid

   #232430

   Purpose

   Expression plasmid for the SpCas9 nuclease with superNLS

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   Cas9–superNLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CMV-VPR-S-L1-dCjCas9 MiniCAFE

   Plasmid

   #169910

   Purpose

   Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   MiniCAFE
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D8A, HNH-truncation (Δ495–609 aa) in CjCas9

   Promoter

   CMV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV_CreNLS_PP7

   Plasmid

   #232439

   Purpose

   expression plasmid to produce Cre-NLS mRNA with a PP7 aptamer in the 3' UTR

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   Cre–MYC-NLS
   

   Expression

   Mammalian

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-SpRYc-ABE8e-EGFP

   Plasmid

   #208340

   Purpose

   Expresses SpRYc-ABE8e in mammalian cells

   Depositor

   Pranam Chatterjee

   Article

   Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

   Insert

   ecTadA(8e)-nSpRYc
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Sc++ (1-1119), SpRY (1111-1368), D10A

   Promoter

   CMV

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CRISPR-SB

   Plasmid

   #177936

   Purpose

   Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

   Depositor

   Roland Rad

   Article

   Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

   Type

   Empty backbone

   Use

   CRISPR; Transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDisplay-GRAB_CRFmut

   Plasmid

   #208656

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Pdpy-30-miniCAFE

    Plasmid

    #170115

    Purpose

    Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    MiniCAFE
    

    Use

    CRISPR

    Mutation

    D8A, HNH-truncation (Δ495–609 aa) in CjCas9

    Promoter

    Dpy-30

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mEGFP-N1-LATS2

    Plasmid

    #166474

    Purpose

    Expresses fusion of mEGFP and LATS2

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, LATS2 (LATS2 Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-EF1_-DIO-GRAB_NPY1.0

    Plasmid

    #208677

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
    

    Use

    AAV

    Promoter

    EF1_

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE-FGFR3__TACC3

    Plasmid

    #116818

    Purpose

    Lentiviral expression of FGFR3__TACC3 fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR3__TACC3
    

    Use

    Lentiviral

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHE111-3xFlag-HSP70

    Plasmid

    #236055

    Purpose

    Mammalian expression of human HSP70 with a N-terminal 3x-FLAG tag

    Depositor

    Jonathan Schisler

    Article

    Stewart et al bioRxiv 2025.02.17.637997

    Insert

    HSP70 (HSPA1A Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDisplay-GRAB_NPY1.0

    Plasmid

    #208675

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. OCT4-eGFP-2A-Puro

    Plasmid

    #31939

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Inserts

    OCT4 (POU5F1 Human)
    eGFP (eGFP )
    

    Use

    Targeting donor

    Tags

    eGFP

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS 3xFLAG Mbnl2

    Plasmid

    #217032

    Purpose

    Overexpression of Mbnl2 in mammalian cells

    Depositor

    Chaolin Zhang

    Article

    Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

    Insert

    Mbnl2 (Mbnl2 Mouse)
    

    Expression

    Mammalian

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSEMS-Tom20-mEGFP-reHaloTagF

    Plasmid

    #200190

    Purpose

    Tom20 with reversible HaloTagF variant for fluorescent labeling

    Depositor

    Jacob Piehler

    Article

    Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

    Insert

    Tom20 (TOMM20 Human)
    

    Tags

    mEGFP and reHaloTagF

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUNIV-SIG-5HTR4D100A

    Plasmid

    #16312

    Depositor

    Bruce Conklin

    Article

    Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

    Insert

    h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (HTR4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Added FLAG tag; Aspartic Acid 100 changed to Alan…

    Available Since

    Jan. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

20. OCT4-eGFP-PGK-Puro

    Plasmid

    #31937

    Purpose

    Oct4 targeting donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge